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Coordonnées
Laboratoire de Bioimagerie et Pathologies
Faculté de Pharmacie
74 route du Rhin
CS 60024
67401 ILLKIRCH Cedex
03 68 85 42 55
Statut
Chercheur
Équipes/plateformes
UMR 7021 - Equipe "Matériaux photoactifs et bioimagerie - MPB"
Publications
Lipid-Directed Covalent Labeling of Plasma Membranes for Long-Term Imaging, Barcoding and Manipulation of Cells.
Aknine N, Pelletier R, Klymchenko AS
JACS Au. 2025 02 24;5(2):922-936
Fluorescent Artificial Receptor for Dopamine based on Molecular Recognition-driven Dynamic Covalent Chemistry in a Lipid Nanoreactor.
Kozibroda B, Lehn JM, Klymchenko AS
Angew Chem Int Ed Engl. 2025 01 13;:e202419905
An exchangeable SIM probe for monitoring organellar dynamics of necrosis cells and intracellular water heterogeneity in kidney repair.
Lai Y, Zhang T, Huang L, Klymchenko AS, Lin W
Proc Natl Acad Sci U S A. 2025 01 7;122(1):e2402348121
POLCAM: instant molecular orientation microscopy for the life sciences.
Bruggeman E, Zhang O, Needham LM, Körbel M, Daly S, Cheetham M, Peters R, Wu T, Klymchenko AS, Davis SJ, Paluch EK, Klenerman D, Lew MD, O'Holleran K, Lee SF
Nat Methods. 2024 10 7;:
FLIM nanoscopy resolves the structure and preferential adsorption in the co-nonsolvency of PNIPAM microgels in methanol-water.
Centeno SP, Nothdurft K, Klymchenko AS, Pich A, Richtering W, Wöll D
J Colloid Interface Sci. 2024 09 2;678(Pt B):210-220
Combining array tomography with electron tomography provides insights into leakiness of the blood-brain barrier in mouse cortex.
Kislinger G, Fabig G, Wehn A, Rodriguez L, Jiang H, Niemann C, Klymchenko AS, Plesnila N, Misgeld T, Müller-Reichert T, Khalin I, Schifferer M
Elife. 2024 08 5;12:
Push-Pull Fluorescent Dyes with Trifluoroacetyl Acceptor for High-Fidelity Sensing of Polarity and Heterogeneity of Lipid Droplets.
Aknine N, Klymchenko AS
Anal Chem. 2024 07 31;:
Direct Zeptomole Detection of RNA Biomarkers by Ultrabright Fluorescent Nanoparticles on Magnetic Beads.
Cruz Da Silva E, Gaki P, Flieg F, Messmer M, Gucciardi F, Markovska Y, Reisch A, Fafi-Kremer S, Pfeffer S, Klymchenko AS
Small. 2024 07 16;:e2404167
Quantum Dot-Based FRET Nanosensors for Talin-Membrane Assembly and Mechanosensing.
Ntadambanya A, Pernier J, David V, Susumu K, Medintz IL, Collot M, Klymchenko A, Hildebrandt N, Le Potier I, Le Clainche C, Cardoso Dos Santos M
Angew Chem Int Ed Engl. 2024 07 15;:e202409852
Exciton annihilation and diffusion length in disordered multichromophoric nanoparticles.
Gharbi AM, Biswas DS, Crégut O, Malý P, Didier P, Klymchenko A, Léonard J
Nanoscale. 2024 06 13;:
To see or not to see: In vivo nanocarrier detection methods in the brain and their challenges.
Wehn AC, Krestel E, Harapan BN, Klymchenko A, Plesnila N, Khalin I
J Control Release. 2024 05 27;:
Fluorescent Solvatochromic Probes for Long-Term Imaging of Lipid Order in Living Cells.
Tanaka T, Matsumoto A, Klymchenko AS, Tsurumaki E, Ikenouchi J, Konishi GI
Adv Sci (Weinh). 2024 03 11;:e2309721
Supramolecular Heterodimer Peptides Assembly for Nanoparticles Functionalization.
Mathieu C, Ghosh S, Draussin J, Gasser A, Jacquot G, Banerjee M, Gupta T, Schmutz M, Mirjolet C, Tillement O, Lux F, Klymchenko A, Donzeau M, Pivot X, Harlepp S, Detappe A
Adv Healthc Mater. 2024 03 5;:e2304250
Fluorescent Probes Based on Charge and Proton Transfer for Probing Biomolecular Environment.
Pivovarenko VG, Klymchenko AS
Chem Rec. 2023 12 29;:e202300321
Mixing versus Polymer Chemistry in the Synthesis of Loaded Polymer Nanoparticles through Nanoprecipitation.
Combes A, Rieb C, Haye L, Klymchenko AS, Serra CA, Reisch A
Langmuir. 2023 11 13;:
Brightness of fluorescent organic nanomaterials.
Ashoka AH, Aparin IO, Reisch A, Klymchenko AS
Chem Soc Rev. 2023 06 20;:
Characterizing Counterion-Dependent Aggregation of Rhodamine B by Classical Molecular Dynamics Simulations.
Fanciullo G, Orlandi S, Klymchenko AS, Muccioli L, Rivalta I
Molecules. 2023 06 13;28(12):
Genetic Targeting of Solvatochromic Dyes for Probing Nanoscale Environments of Proteins in Organelles.
Pelletier R, Danylchuk DI, Benaissa H, Broch F, Vauchelles R, Gautier A, Klymchenko AS
Anal Chem. 2023 05 25;:
Long-range Energy Transfer Between Dye-loaded Nanoparticles: Observation and Amplified Detection of Nucleic Acids.
Biswas DS, Gaki P, Da Silva EC, Combes A, Reisch A, Didier P, Klymchenko AS
Adv Mater. 2023 04 18;:e2301402
Tuning Directed Photooxidation-Induced Conversion of Pyrrole-Based Styryl Coumarin Dual-Color Photoconverters.
Saladin L, Dal Pra O, Klymchenko AS, Didier P, Collot M
Chemistry. 2023 03 15;:e202300685
Biotinylated Fluorescent Polymeric Nanoparticles for Enhanced Immunostaining.
Yudhistira T, Da Silva EC, Combes A, Lehmann M, Reisch A, Klymchenko AS
Small Methods. 2023 02 19;:e2201452
Tuning Directed Photooxidation Induced Conversion of Pyrrole-based Styryl Coumarins Dual-Color Photoconverters.
Saladin L, Dal Pra O, Klymchenko A, Didier P, Collot M
Chemistry. 2023 01 31;:
The Plasma Membrane Polarity Is Higher in the Neuronal Growth Cone than in the Cell Body of Hippocampal and Cerebellar Granule Neurons.
Oya S, Korogi K, Kohno T, Tsuiji H, Danylchuk DI, Klymchenko AS, Niko Y, Hattori M
Biol Pharm Bull. 2023 ;46(12):1820-1825
Fluorescent Probes for Lipid Membranes: From the Cell Surface to Organelles.
Klymchenko AS
Acc Chem Res. 2022 12 19;:
Study of surfactant cross-linking by click chemistry on a model water/oil interface.
Brou GA, Gbassi GK, Shulov I, Seralin A, Klymchenko AS, Vandamme TF, Anton N
Phys Chem Chem Phys. 2022 12 15;:
Controlled Release and Capture of Aldehydes by Dynamic Imine Chemistry in Nanoemulsions: From Delivery to Detoxification.
Liu F, Anton N, Niko Y, Klymchenko AS
ACS Appl Bio Mater. 2022 12 14;:
Intracellular accumulation and immunological response of NIR-II polymeric nanoparticles.
Moskalevska I, Faure V, Haye L, Mercey-Ressejac M, Dey AK, Chovelon B, Soro LK, Charbonnière LJ, Reisch A, Klymchenko AS, Marche PN, Coll JL, Macek Jilkova Z, le Guével X
Int J Pharm. 2022 11 26;630:122439
Dual-Color Photoconvertible Fluorescent Probes Based on Directed Photooxidation Induced Conversion for bioimaging.
Saladin L, Breton V, Dal Pra O, Klymchenko AS, Danglot L, Didier P, Collot M
Angew Chem Int Ed Engl. 2022 11 24;:
Fluorogenic Dimers as Bright Switchable Probes for Enhanced Super-Resolution Imaging of Cell Membranes.
Aparin IO, Yan R, Pelletier R, Choi AA, Danylchuk DI, Xu K, Klymchenko AS
J Am Chem Soc. 2022 09 26;:
Engineering Novel 3D Models to Recreate High-Grade Osteosarcoma and its Immune and Extracellular Matrix Microenvironment.
Pierrevelcin M, Flacher V, Mueller CG, Vauchelles R, Guerin E, Lhermitte B, Pencreach E, Reisch A, Muller Q, Doumard L, Boufenghour W, Klymchenko AS, Foppolo S, Nazon C, Weingertner N, Martin S, Briandet C, Laithier V, Di Marco A, Bund L, Obrecht A, Villa P, Dontenwill M, Entz-Werlé N
Adv Healthc Mater. 2022 09 4;:e2200195
Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties.
Ragaller F, Andronico L, Sykora J, Kulig W, Rog T, Urem YB, Abhinav,Danylchuk DI, Hof M, Klymchenko A, Amaro M, Vattulainen I, Sezgin E
Open Biol. 2022 09;12(9):220175
In Vivo Imaging Evaluation of Fluorescence Intensity at Tail Emission of Near-Infrared-I (NIR-I) Fluorophores in a Porcine Model.
Rodríguez-Luna MR, Okamoto N, Al-Taher M, Keller DS, Cinelli L, Hoskere Ashoka A, Klymchenko AS, Marescaux J, Diana M
Life (Basel). 2022 07 27;12(8):
Assembly of Fluorescent Polymer Nanoparticles Using Different Microfluidic Mixers.
Chen H, Celik AE, Mutschler A, Combes A, Runser A, Klymchenko AS, Lecommandoux S, Serra CA, Reisch A
Langmuir. 2022 06 22;:
Rational Design of Self-Quenched Rhodamine Dimers as Fluorogenic Aptamer Probes for Live-Cell RNA Imaging.
Fam KT, Pelletier R, Bouhedda F, Ryckelynck M, Collot M, Klymchenko AS
Anal Chem. 2022 Apr 29;:
Size-Selective Transfer of Lipid Nanoparticle-Based Drug Carriers Across the Blood Brain Barrier Via Vascular Occlusions Following Traumatic Brain Injury.
Khalin I, Adarsh N, Schifferer M, Wehn A, Groschup B, Misgeld T, Klymchenko A, Plesnila N
Small. 2022 Apr 5;:e2200302
Imaging and Measuring Vesicular Acidification with a Plasma Membrane-Targeted Ratiometric pH Probe.
Michelis S, Danglot L, Vauchelles R, Klymchenko AS, Collot M
Anal Chem. 2022 Apr 4;:
Dynamic covalent chemistry in live cells for organelle targeting and enhanced photodynamic action.
Liu F, Danylchuk DI, Andreiuk B, Klymchenko AS
Chem Sci. 2022 Mar 30;13(13):3652-3660
Cardiac forces regulate zebrafish heart valve delamination by modulating Nfat signaling.
Chow RW, Fukui H, Chan WX, Tan KSJ, Roth S, Duchemin AL, Messaddeq N, Nakajima H, Liu F, Faggianelli-Conrozier N, Klymchenko AS, Choon Hwai Y, Mochizuki N, Vermot J
PLoS Biol. 2022 Jan 14;20(1):e3001505
Modelling quenching mechanisms of disordered molecular systems in the presence of molecular aggregates.
Fanciullo G, Conti I, Didier P, Klymchenko A, Léonard J, Garavelli M, Rivalta I
Phys Chem Chem Phys. 2022 Jan 5;:
Counterion-insulated near-infrared dyes in biodegradable polymer nanoparticles for imaging.
Sobska J, Andreiuk B, Aparin IO, Reisch A, Krezel W, Klymchenko AS
Nanoscale Adv. 2021 Dec 21;4(1):39-48
Amplified Fluorescence Hybridization by Small and Bright Dye-Loaded Polymeric Nanoparticles.
Egloff S, Melnychuk N, Cruz Da Silva E, Reisch A, Martin S, Klymchenko AS
ACS Nano. 2021 Dec 20;:
Dynamic tracing using ultra-bright labelling and multi-photon microscopy identifies endothelial uptake of poloxamer 188 coated poly(lactic-co-glycolic acid) nano-carriers in vivo.
Khalin I, Severi C, Heimburger D, Wehn A, Hellal F, Reisch A, Klymchenko AS, Plesnila N
Nanomedicine. 2021 Dec 13;:102511
Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes.
Carravilla P, Dasgupta A, Zhurgenbayeva G, Danylchuk DI, Klymchenko AS, Sezgin E, Eggeling C
Biophys Rep. 2021 Dec 8;1(2):None
Protein-like particles through nanoprecipitation of mixtures of polymers of opposite charge.
Combes A, Tang KN, Klymchenko AS, Reisch A
J Colloid Interface Sci. 2021 Sep 20;607(Pt 2):1786-1795
Probing Variations of Reduction Activity at the Plasma Membrane Using a Targeted Ratiometric FRET Probe.
Mukherjee T, Kanvah S, Klymchenko AS, Collot M
ACS Appl Mater Interfaces. 2021 Aug 23;:
Emerging Solvatochromic Push-Pull Dyes for Monitoring the Lipid Order of Biomembranes in Live Cells.
Niko Y, Klymchenko AS
J Biochem. 2021 Jul 2;:
Bulky Barbiturates as Non-toxic Ionic Dye Insulators for Enhanced Emission in Polymeric Nanoparticles.
Andreiuk B, Aparin I, Reisch A, Klymchenko AS
Chemistry. 2021 Jun 23;:
Ultrabright Fluorescent Polymeric Nanofibers and Coatings Based on Ionic Dye Insulation with Bulky Counterions.
Ashoka AH, Klymchenko AS
ACS Appl Mater Interfaces. 2021 Jun 9;:
Molecular organization in hydroperoxidized POPC bilayers.
Junqueira H, Schroder AP, Thalmann F, Klymchenko A, Mély Y, Baptista MS, Marques CM
Biochim Biophys Acta Biomembr. 2021 May 27;:183659
Confronting molecular rotors and self-quenched dimers as fluorogenic BODIPY systems to probe biotin receptors in cancer cells.
Fam KT, Saladin L, Klymchenko AS, Collot M
Chem Commun (Camb). 2021 May 13;57(39):4807-4810
µIVC-Useq: a microfluidic-assisted high-throughput functionnal screening in tandem with next generation sequencing and artificial neural network to rapidly characterize RNA molecules.
Cubi R, Bouhedda F, Collot M, Klymchenko AS, Ryckelynck M
RNA. 2021 May 5;:
Fluorescent labeling of biocompatible block copolymers: synthetic strategies and applications in bioimaging.
Bou S, Klymchenko AS, Collot M
Mater Adv. 2021 Apr 6;2(10):3213-3233
Ultrabright Green-Emitting Nanoemulsions Based on Natural Lipids-BODIPY Conjugates.
Wang X, Bou S, Klymchenko AS, Anton N, Collot M
Nanomaterials (Basel). 2021 Mar 23;11(3):
Nile Red-Based GPCR Ligands as Ultrasensitive Probes of the Local Lipid Microenvironment of the Receptor.
Hanser F, Marsol C, Valencia C, Villa P, Klymchenko AS, Bonnet D, Karpenko J
ACS Chem Biol. 2021 Mar 18;:
Fluorescent nanocarriers targeting VCAM-1 for early detection of senescent endothelial cells.
Belcastro E, Rehman AU, Remila L, Park SH, Gong DS, Anton N, Auger C, Lefebvre O, Goetz JG, Collot M, Klymchenko AS, Vandamme TF, Schini-Kerth VB
Nanomedicine. 2021 Mar 10;:102379
Intraoperative ureter identification with a novel fluorescent catheter.
Barberio M, Al-Taher M, Felli E, Ashoka AH, Marescaux J, Klymchenko A, Diana M
Sci Rep. 2021 Feb 24;11(1):4501
Enzyme-free amplified detection of cellular microRNA by light-harvesting fluorescent nanoparticle probes.
Egloff S, Melnychuk N, Reisch A, Martin S, Klymchenko AS
Biosens Bioelectron. 2021 Feb 10;179:113084
Fluorogenic squaraine dendrimers for background-free imaging of integrin receptors in cancer cells.
Ashokkumar P, Collot M, Klymchenko AS
Chemistry. 2021 Feb 10;:
Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe.
Mukherjee T, Soppina V, Ludovic R, Mély Y, Klymchenko AS, Collot M, Kanvah S
Org Biomol Chem. 2021 Feb 8;:
Size-Dependent Electroporation of Dye-Loaded Polymer Nanoparticles for Efficient and Safe Intracellular Delivery.
Egloff S, Runser A, Klymchenko A, Reisch A
Small Methods. 2021 Feb;5(2):e2000947
Targeted Solvatochromic Fluorescent Probes for Imaging Lipid Order in Organelles under Oxidative and Mechanical Stress.
Danylchuk DI, Jouard PH, Klymchenko AS
J Am Chem Soc. 2021 Jan 8;:
Tunable functionalization of nano-emulsions using amphiphilic polymers.
Rehman AU, Anton N, Bou S, Schild J, Messaddeq N, Vandamme T, Akram S, Klymchenko A, Collot M
Soft Matter. 2021 Jan 5;:
Microcavity-Enhanced Fluorescence Energy Transfer from Quantum Dot Excited Whispering Gallery Modes to Acceptor Dye Nanoparticles.
Jana S, Xu X, Klymchenko A, Reisch A, Pons T
ACS Nano. 2020 Dec 30;:
Time-resolved MIET measurements of blood platelet spreading and adhesion.
Zelená A, Isbaner S, Ruhlandt D, Chizhik A, Cassini C, Klymchenko AS, Enderlein J, Chizhik A, Köster S
Nanoscale. 2020 Oct 19;:
Dye-Loaded Nanoemulsions: Biomimetic Fluorescent Nanocarriers for Bioimaging and Nanomedicine.
Klymchenko AS, Liu F, Collot M, Anton N
Adv Healthc Mater. 2020 Oct 13;:e2001289
Redesigning Solvatochromic Probe Laurdan for Imaging Lipid Order Selectively in Cell Plasma Membranes.
Danylchuk DI, Sezgin E, Chabert P, Klymchenko AS
Anal Chem. 2020 Oct 12;:
Stealth and Bright Monomolecular Fluorescent Organic Nanoparticles Based on Folded Amphiphilic Polymer.
Collot M, Schild J, Fam KT, Bouchaala R, Klymchenko AS
ACS Nano. 2020 Oct 6;:
Preoperative endoscopic marking of the gastrointestinal tract using fluorescence imaging: submucosal indocyanine green tattooing versus a novel fluorescent over-the-scope clip in a survival experimental study.
Barberio M, Pizzicannella M, Spota A, Ashoka AH, Agnus V, Al Taher M, Jansen-Winkeln B, Gockel I, Marescaux J, Swanström L, Kong SH, Felli E, Klymchenko A, Diana M
Surg Endosc. 2020 Sep 28;:
Phase-selective staining of model and cell membranes, lipid droplets and lipoproteins with fluorescent solvatochromic pyrene probes.
Sot J, Esnal I, Monasterio BG, León-Irra R, Niko Y, Goñi FM, Klymchenko A, Alonso A
Biochim Biophys Acta Biomembr. 2020 Sep 5;:183470
Simultaneous multipurpose fluorescence imaging with IRDye® 800BK during laparoscopic surgery.
Al-Taher M, Barberio M, Felli E, Agnus V, Ashoka AH, Gioux S, Klymchenko A, Bouvy N, Stassen L, Marescaux J, Diana M
Surg Endosc. 2020 Aug 28;:
Near-infrared fluorescent coatings of medical devices for image-guided surgery.
Ashoka AH, Kong SH, Seeliger B, Andreiuk B, Soares RV, Barberio M, Diana M, Klymchenko AS
Biomaterials. 2020 Aug 20;261:120306
Smartphone-assisted detection of nucleic acids by light-harvesting FRET-based nanoprobe.
Severi C, Melnychuk N, Klymchenko AS
Biosens Bioelectron. 2020 Aug 15;168:112515
Ultrabright Fluorescent Polymeric Nanoparticles with Stealth Pluronic Shell for Live Tracking in Mouse Brain.
Khalin I, Heimburger D, Melnychuk N, Collot M, Groschup B, Hellal F, Reisch A, Plesnila N, Klymchenko AS
ACS Nano. 2020 Jul 17;:
Near infrared fluorogenic probe as a prodrug model for evaluating cargo release by nanoemulsions.
Bou S, Wang X, Anton N, Klymchenko AS, Collot M
J Mater Chem B. 2020 Jul 15;8(27):5938-5944
A near-infrared fluorogenic dimer enables background-free imaging of endogenous GPCRs in living mice.
Esteoulle L, Daubeuf F, Collot M, Riché S, Durroux T, Brasse D, Marchand P, Karpenko IA, Klymchenko AS, Bonnet D
Chem Sci. 2020 Jul 14;11(26):6824-6829
Probing biotin receptors in cancer cells with rationally designed fluorogenic squaraine dimers.
Fam KT, Collot M, Klymchenko AS
Chem Sci. 2020 Jul 9;11(31):8240-8248
Ratiometric Nanoparticle Probe Based on FRET-Amplified Phosphorescence for Oxygen Sensing with Minimal Phototoxicity.
Ashokkumar P, Adarsh N, Klymchenko AS
Small. 2020 Jun 25;:e2002494
Further insights into release mechanisms from nano-emulsions, assessed by a simple fluorescence-based method.
Wang X, Collot M, Omran Z, Vandamme TF, Klymchenko A, Anton N
J Colloid Interface Sci. 2020 Jun 11;578:768-778
Development of a thermosensitive statin loaded chitosan-based hydrogel promoting bone healing.
Petit C, Batool F, Stutz C, Anton N, Klymchenko A, Vandamme T, Benkirane-Jessel N, Huck O
Int J Pharm. 2020 Jun 9;:119534
A novel method for near-infrared fluorescence imaging of the urethra during perineal and transanal surgery: demonstration in a cadaveric model.
Barberio M, Al-Taher M, Forgione A, Hoskere Ashoka A, Felli E, Agnus V, Marescaux J, Klymchenko A, Diana M
Colorectal Dis. 2020 May 22;:
Lipid-core/polymer-shell hybrid nanoparticles: synthesis and characterization by fluorescence labeling and electrophoresis.
Bou S, Wang X, Anton N, Bouchaala R, Klymchenko AS, Collot M
Soft Matter. 2020 Apr 14;:
Polarity mapping of cells and embryos by improved fluorescent solvatochromic pyrene probe.
Valanciunaite J, Kempf E, Seki H, Danylchuk DI, Peyriéras N, Niko Y, Klymchenko AS
Anal. Chem.. 2020 Mar 10;:
Molecular Tuning of Styryl Dyes Leads to Versatile and Efficient Plasma Membrane Probes for Cell and Tissue Imaging.
Collot M, Boutant E, Fam KT, Danglot L, Klymchenko AS
Bioconjug. Chem.. 2020 Feb 13;:
Light-harvesting nanoparticle probes for FRET-based detection of oligonucleotides with single-molecule sensitivity.
Melnychuk N, Egloff S, Runser A, Reisch A, Klymchenko AS
Angew. Chem. Int. Ed. Engl.. 2020 Jan 15;:
Zwitterionic Stealth Dye-Loaded Polymer Nanoparticles for Intracellular Imaging.
Runser A, Dujardin D, Ernst P, Klymchenko AS, Reisch A
ACS Appl Mater Interfaces. 2019 Dec 24;:
Simultaneous computer-assisted assessment of mucosal and serosal perfusion in a model of segmental colonic ischemia.
Seeliger B, Agnus V, Mascagni P, Barberio M, Longo F, Lapergola A, Mutter D, Klymchenko AS, Chand M, Marescaux J, Diana M
Surg Endosc. 2019 Nov 18;:
A dimerization-based fluorogenic dye-aptamer module for RNA imaging in live cells.
Bouhedda F, Fam KT, Collot M, Autour A, Marzi S, Klymchenko A, Ryckelynck M
Nat. Chem. Biol.. 2019 Oct 21;:
Switchable Solvatochromic Probes for Live-Cell Super-resolution Imaging of Plasma Membrane Organization.
Danylchuk DI, Moon S, Xu K, Klymchenko AS
Angew. Chem. Int. Ed. Engl.. 2019 Oct 14;58(42):14920-14924
-Palmitoylation of junctophilin-2 is critical for its role in tethering the sarcoplasmic reticulum to the plasma membrane.
Jiang M, Hu J, White FKH, Williamson J, Klymchenko AS, Murthy A, Workman SW, Tseng GN
J. Biol. Chem.. 2019 Sep 6;294(36):13487-13501
BODIPY-loaded polymer nanoparticles: chemical structure of cargo defines leakage from nanocarrier in living cells.
Trofymchuk K, Valanciunaite J, Andreiuk B, Reisch A, Collot M, Klymchenko AS
J Mater Chem B. 2019 Aug 28;7(34):5199-5210
Ionic aggregation-induced emission dye with bulky counterions for preparation of bright near-infrared polymeric nanoparticles.
Adarsh N, Klymchenko AS
Nanoscale. 2019 Aug 7;11(29):13977-13987
Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells.
Buenaventura T, Bitsi S, Laughlin WE, Burgoyne T, Lyu Z, Oqua AI, Norman H, McGlone ER, Klymchenko AS, Corrêa IR, Walker A, Inoue A, Hanyaloglu A, Grimes J, Koszegi Z, Calebiro D, Rutter GA, Bloom SR, Jones B, Tomas A
PLoS Biol.. 2019 Aug;17(8):e3000097
A fluorogenic BODIPY molecular rotor as an apoptosis marker.
Ashokkumar P, Ashoka AH, Collot M, Das A, Klymchenko AS
Chem. Commun. (Camb.). 2019 Jun 11;55(48):6902-6905
Solvatochromic Near-Infrared Probe for Polarity Mapping of Biomembranes and Lipid Droplets in Cells under Stress.
Ashoka AH, Ashokkumar P, Kovtun YP, Klymchenko AS
J Phys Chem Lett. 2019 May 16;10(10):2414-2421
Controlling Size and Fluorescence of Dye-Loaded Polymer Nanoparticles through Polymer Design.
Rosiuk V, Runser A, Klymchenko A, Reisch A
Langmuir. 2019 May 13;:
Optimizing the Fluorescence Properties of Nanoemulsions for Single Particle Tracking in Live Cells.
Wang X, Anton N, Ashokkumar P, Anton H, Fam TK, Vandamme T, Klymchenko AS, Collot M
ACS Appl Mater Interfaces. 2019 Apr 10;11(14):13079-13090
Fighting Aggregation-Caused Quenching and Leakage of Dyes in Fluorescent Polymer Nanoparticles: Universal Role of Counterion.
Andreiuk B, Reisch A, Bernhardt E, Klymchenko AS
Chem Asian J. 2019 Mar 15;14(6):836-846
MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience.
Collot M, Ashokkumar P, Anton H, Boutant E, Faklaris O, Galli T, Mély Y, Danglot L, Klymchenko AS
Cell Chem Biol. 2019 Feb 6;:
Probing Polarity and Heterogeneity of Lipid Droplets in Live Cells Using a Push-Pull Fluorophore.
Collot M, Bou S, Fam TK, Richert L, Mély Y, Danglot L, Klymchenko AS
Anal. Chem.. 2019 Feb 5;91(3):1928-1935
BODIPY with Tuned Amphiphilicity as a Fluorogenic Plasma Membrane Probe.
Collot M, Boutant E, Lehmann M, Klymchenko AS
Bioconjug. Chem.. 2019 01 16;30(1):192-199
Recent Advances in Fluorescent Probes for Lipid Droplets.
Fam TK, Klymchenko AS, Collot M
Materials (Basel). 2018 Sep 18;11(9):
DNA-Functionalized Dye-Loaded Polymeric Nanoparticles: Ultrabright FRET Platform for Amplified Detection of Nucleic Acids.
Melnychuk N, Klymchenko AS
J. Am. Chem. Soc.. 2018 08 29;140(34):10856-10865
Apoptosis and eryptosis: Striking differences on biomembrane level.
Pyrshev KA, Klymchenko AS, Csúcs G, Demchenko AP
Biochim Biophys Acta Biomembr. 2018 Jun;1860(6):1362-1371
Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues.
Collot M, Fam TK, Ashokkumar P, Faklaris O, Galli T, Danglot L, Klymchenko AS
J. Am. Chem. Soc.. 2018 04 25;140(16):5401-5411
In Vivo FRET Imaging to Predict the Risk Associated with Hepatic Accumulation of Squalene-Based Prodrug Nanoparticles.
Cayre F, Mura S, Andreiuk B, Sobot D, Gouazou S, Desmaële D, Klymchenko AS, Couvreur P
Adv Healthc Mater. 2018 02;7(3):
Tailoring Fluorescence Brightness and Switching of Nanoparticles through Dye Organization in the Polymer Matrix.
Reisch A, Trofymchuk K, Runser A, Fleith G, Rawiso M, Klymchenko AS
ACS Appl Mater Interfaces. 2017 Dec 13;9(49):43030-43042
PEGylated Red-Emitting Calcium Probe with Improved Sensing Properties for Neuroscience.
Ponsot F, Shen W, Ashokkumar P, Audinat E, Klymchenko AS, Collot M
ACS Sens. 2017 11 22;2(11):1706-1712
Giant light-harvesting nanoantenna for single-molecule detection in ambient light.
Trofymchuk K, Reisch A, Didier P, Fras F, Gilliot P, Mely Y, Klymchenko AS
Nat Photonics. 2017 Oct;11(10):657-663
Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy.
Sezgin E, Schneider F, Zilles V, Urbančič I, Garcia E, Waithe D, Klymchenko AS, Eggeling C
Biophys. J.. 2017 Sep 19;113(6):1321-1330
Labeling nanoparticles: Dye leakage and altered cellular uptake.
Snipstad S, Hak S, Baghirov H, Sulheim E, Mørch Ý, Lélu S, von Haartman E, Bäck M, Nilsson KPR, Klymchenko AS, de Lange Davies C, Åslund AKO
Cytometry A. 2017 08;91(8):760-766
Quantitative assessment of energy transfer in upconverting nanoparticles grafted with organic dyes.
Dukhno O, Przybilla F, Collot M, Klymchenko A, Pivovarenko V, Buchner M, Muhr V, Hirsch T, Mély Y
Nanoscale. 2017 Aug;:
Functionalizing Nanoemulsions with Carboxylates: Impact on the Biodistribution and Pharmacokinetics in Mice.
Attia MF, Dieng SM, Collot M, Klymchenko AS, Bouillot C, Serra CA, Schmutz M, Er-Rafik M, Vandamme TF, Anton N
Macromol Biosci. 2017 07;17(7):
Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore.
Collot M, Ponsot F, Klymchenko AS
Chem. Commun. (Camb.). 2017 Jun 1;53(45):6117-6120
Conjugation of squalene to gemcitabine as unique approach exploiting endogenous lipoproteins for drug delivery.
Sobot D, Mura S, Yesylevskyy SO, Dalbin L, Cayre F, Bort G, Mougin J, Desmaële D, Lepetre-Mouelhi S, Pieters G, Andreiuk B, Klymchenko AS, Paul JL, Ramseyer C, Couvreur P
Nat Commun. 2017 05 30;8:15678
Lipid nanocapsules maintain full integrity after crossing a human intestinal epithelium model.
Roger E, Gimel JC, Bensley C, Klymchenko AS, Benoit JP
J Control Release. 2017 05 10;253:11-18
Light-triggered release from dye-loaded fluorescent lipid nanocarriers in vitro and in vivo.
Bouchaala R, Anton N, Anton H, Vandamme T, Vermot J, Smail D, Mély Y, Klymchenko AS
Colloids Surf B Biointerfaces. 2017 May;156:414-421
Visualising the membrane viscosity of porcine eye lens cells using molecular rotors.
Sherin PS, López-Duarte I, Dent MR, Kubánková M, Vyšniauskas A, Bull JA, Reshetnikova ES, Klymchenko AS, Tsentalovich YP, Kuimova MK
Chem Sci. 2017 May 1;8(5):3523-3528
A new method for the formulation of double nanoemulsions.
Ding S, Anton N, Akram S, Er-Rafik M, Anton H, Klymchenko A, Yu W, Vandamme TF, Serra CA
Soft Matter. 2017 Feb 22;13(8):1660-1669
Solvatochromic and Fluorogenic Dyes as Environment-Sensitive Probes: Design and Biological Applications.
Klymchenko AS
Acc. Chem. Res.. 2017 02 21;50(2):366-375
Protein-Sized Bright Fluorogenic Nanoparticles Based on Cross-Linked Calixarene Micelles with Cyanine Corona.
Shulov I, Rodik RV, Arntz Y, Reisch A, Kalchenko VI, Klymchenko AS
Angew. Chem. Int. Ed. Engl.. 2016 12 19;55(51):15884-15888
Robust augmented reality registration method for localization of solid organs' tumors using CT-derived virtual biomechanical model and fluorescent fiducials.
Kong SH, Haouchine N, Soares R, Klymchenko A, Andreiuk B, Marques B, Shabat G, Piechaud T, Diana M, Cotin S, Marescaux J
Surg Endosc. 2016 Oct;:
Non-coordinating anions assemble cyanine amphiphiles into ultra-small fluorescent nanoparticles.
Shulov I, Arntz Y, Mély Y, Pivovarenko VG, Klymchenko AS
Chem. Commun. (Camb.). 2016 Jun;52(51):7962-5
Push-pull dioxaborine as fluorescent molecular rotor: far-red fluorogenic probe for ligand-receptor interactions.
Karpenko IA, Niko Y, Yakubovskyi VP, Gerasov AO, Bonnet D, Kovtun YP, Klymchenko AS
J Mater Chem C Mater. 2016 Apr 14;4(14):3002-3009
Fluorescent Polymer Nanoparticles Based on Dyes: Seeking Brighter Tools for Bioimaging.
Reisch A, Klymchenko AS
Small. 2016 Apr;12(15):1968-92
Neuronal Uptake and Neuroprotective Properties of Curcumin-Loaded Nanoparticles on SK-N-SH Cell Line: Role of Poly(lactide-co-glycolide) Polymeric Matrix Composition.
Djiokeng Paka G, Doggui S, Zaghmi A, Safar R, Dao L, Reisch A, Klymchenko A, Roullin VG, Joubert O, Ramassamy C
Mol. Pharm.. 2016 Feb 1;13(2):391-403
Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular membranes.
Niko Y, Didier P, Mely Y, Konishi G, Klymchenko AS
Sci Rep. 2016 Jan;6:18870
Microfluidic conceived Trojan microcarriers for oral delivery of nanoparticles.
Khan IU, Serra CA, Anton N, Er-Rafik M, Blanck C, Schmutz M, Kraus I, Messaddeq N, Sutter C, Anton H, Klymchenko AS, Vandamme TF
Int J Pharm. 2015 Sep 30;493(1-2):7-15
Photopolymerized micelles of diacetylene amphiphile: physical characterization and cell delivery properties.
Neuberg P, Perino A, Morin-Picardat E, Anton N, Darwich Z, Weltin D, Mely Y, Klymchenko AS, Remy JS, Wagner A
Chem. Commun. (Camb.). 2015 Jul;51(58):11595-8
Solvatochromic Nile Red probes with FRET quencher reveal lipid order heterogeneity in living and apoptotic cells.
Kreder R, Pyrshev KA, Darwich Z, Kucherak OA, Mély Y, Klymchenko AS
ACS Chem. Biol.. 2015 Jun;10(6):1435-42
Fluorescence lifetime imaging of membrane lipid order with a ratiometric fluorescent probe.
Kilin V, Glushonkov O, Herdly L, Klymchenko A, Richert L, Mely Y
Biophys J. 2015 May 19;108(10):2521-31
Disassembly-driven fluorescence turn-on of polymerized micelles by reductive stimuli in living cells.
Niko Y, Arntz Y, Mely Y, Konishi G, Klymchenko AS
Chemistry. 2014 Dec;20(50):16473-7
Fluorogenic Squaraine Dimers with Polarity-Sensitive Folding As Bright Far-Red Probes for Background-Free Bioimaging.
Karpenko IA, Collot M, Richert L, Valencia C, Villa P, Mely Y, Hibert M, Bonnet D, Klymchenko AS
J Am Chem Soc. 2014 Dec 29.
Tuning the color and photostability of perylene diimides inside polymer nanoparticles: towards biodegradable substitutes of quantum dots.
Trofymchuk K, Reisch A, Shulov I, Mély Y, Klymchenko AS
Nanoscale. 2014 Nov;6(21):12934-42
Biodistribution of X-ray iodinated contrast agent in nano-emulsions is controlled by the chemical nature of the oily core.
Attia MF, Anton N, Chiper M, Akasov R, Anton H, Messaddeq N, Fournel S, Klymchenko AS, Mely Y, Vandamme TF
ACS Nano. 2014 Oct 28;8(10):10537-50
Fluorescent Amino Acid Undergoing Excited State Intramolecular Proton Transfer for Site-Specific Probing and Imaging of Peptide Interactions.
Sholokh M, Zamotaiev OM, Das R, Postupalenko VY, Richert L, Dujardin D, Zaporozhets OA, Pivovarenko VG, Klymchenko AS, Mely Y
J Phys Chem B. 2014 Oct 22.
Location, dynamics and solvent relaxation of a Nile Red-based phase-sensitive fluorescent membrane probe.
Saxena R, Shrivastava S, Haldar S, Klymchenko AS, Chattopadhyay A
Chem. Phys. Lipids. 2014 Oct;183:1-8
Counterion-enhanced cyanine dye loading into lipid nano-droplets for single-particle tracking in zebrafish.
Kilin VN, Anton H, Anton N, Steed E, Vermot J, Vandamme TF, Mely Y, Klymchenko AS
Biomaterials. 2014 Jun;35(18):4950-7
Poly-epsilon-caprolactone tungsten oxide nanoparticles as a contrast agent for X-ray computed tomography.
Jakhmola A, Anton N, Anton H, Messaddeq N, Hallouard F, Klymchenko A, Mely Y, Vandamme TF
Biomaterials. 2014 Mar;35(9):2981-6
Rational design of a solvatochromic fluorescent uracil analogue with a dual-band ratiometric response based on 3-hydroxychromone.
Dziuba D, Karpenko IA, Barthes NP, Michel BY, Klymchenko AS, Benhida R, Demchenko AP, Mely Y, Burger A
Chemistry. 2014 Feb 10;20(7):1998-2009
Collective fluorescence switching of counterion-assembled dyes in polymer nanoparticles.
Reisch A, Didier P, Richert L, Oncul S, Arntz Y, Mely Y, Klymchenko AS
Nat Commun. 2014 Jun 9;5:4089
Tuning excited-state proton transfer dynamics of a 3-hydroxychromone dye in supramolecular complexes via host-guest steric compatibility.
Das R, Duportail G, Ghose A, Richert L, Klymchenko A, Chakraborty S, Yesylevskyy S, Mely Y
Phys Chem Chem Phys. 2014 Jan 14;16(2):776-84
Detection of apoptosis through the lipid order of the outer plasma membrane leaflet.
Darwich Z, Klymchenko AS, Kucherak OA, Richert L, Mely Y
Biochim Biophys Acta. 2012 Dec;1818(12):3048-54
A universal nucleoside with strong two-band switchable fluorescence and sensitivity to the environment for investigating DNA interactions.
Dziuba D, Postupalenko VY, Spadafora M, Klymchenko AS, Guerineau V, Mely Y, Benhida R, Burger A
J Am Chem Soc. 2012 Jun 20;134(24):10209-13
Sensing micelle hydration by proton-transfer dynamics of a 3-hydroxychromone dye: role of the surfactant headgroup and chain length.
Das R, Duportail G, Richert L, Klymchenko A, Mely Y
Langmuir. 2012 May 8;28(18):7147-59
Dipolar 3-methoxychromones as bright and highly solvatochromic fluorescent dyes.
Kucherak OA, Richert L, Mely Y, Klymchenko AS
Phys Chem Chem Phys. 2012 Feb 21;14(7):2292-300
Highly lipophilic fluorescent dyes in nano-emulsions: towards bright non-leaking nano-droplets.
Klymchenko AS, Roger E, Anton N, Anton H, Shulov I, Vermot J, Mely Y, Vandamme TF
. 2012;2(31):11876-86.
Liquid ordered phase in cell membranes evidenced by a hydration-sensitive probe: effects of cholesterol depletion and apoptosis.
Oncul S, Klymchenko AS, Kucherak OA, Demchenko AP, Martin S, Dontenwill M, Arntz Y, Didier P, Duportail G, Mely Y
Biochim Biophys Acta. 2010 Jul;1798(7):1436-43
Switchable Nile Red-Based Probe for Cholesterol and Lipid Order at the Outer Leaflet of Biomembranes.
Kucherak OA, Oncul S, Darwich Z, Yushchenko DA, Arntz Y, Didier P, Mely Y, Klymchenko AS
J Am Chem Soc. 2010 Apr 7;132(13):4907-16
Fluorene Analogues of Prodan with Superior Fluorescence Brightness and Solvatochromism.
Kucherak OA, Didier P, Mely Y, Klymchenko AS
J Phys Chem Lett. 2010 Feb 4;1(3):616-20.
Visualization of lipid domains in giant unilamellar vesicles using an environment-sensitive membrane probe based on 3-hydroxyflavone.
Klymchenko AS, Oncul S, Didier P, Schaub E, Bagatolli L, Duportail G, Mely Y
Biochim Biophys Acta. 2009 Feb;1788(2):495-9
