Evidence that the (6-4) photolyase mechanism can proceed through an oxetane intermediate.

Date publication

septembre 2008

Auteurs

Membres identifiés du Cancéropôle Est :
Pr GUILLAUME Dominique

Tous les auteurs :
Asgatay S, Petermann C, Harakat D, Guillaume D, Taylor JS, Clivio P

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/18763765

Résumé

Using the analogue of TpT methylated at the 3'-end N3 position (Tpm3T), we demonstrate that when the oxetane/(6-4) pathway is precluded, water addition occurs at the 3'-end C6 position of the oxetane intermediate to provide its opening. Photoreversal of this (6-4) photoproduct C6 hydrate brings the first experimental evidence that the (6-4) photolyase repair can proceed through an oxetane intermediate.

Référence

J Am Chem Soc. 2008 Sep 24;130(38):12618-9