The Drosophila NURF remodelling and the ATAC histone acetylase complexes functionally interact and are required for global chromosome organization.

Date publication

février 2008

Auteurs

Membres identifiés du Cancéropôle Est :
Dr TORA Laszlo

Tous les auteurs :
Carre C, Ciurciu A, Komonyi O, Jacquier C, Fagegaltier D, Pidoux J, Tricoire H, Tora L, Boros IM, Antoniewski C

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/18084186

Résumé

Drosophila Gcn5 is the catalytic subunit of the SAGA and ATAC histone acetylase complexes. Here, we show that mutations in Gcn5 and the ATAC component Ada2a induce a decondensation of the male X chromosome, similar to that induced by mutations in the Iswi and Nurf301 subunits of the NURF nucleosome remodelling complex. Genetic studies as well as transcript profiling analysis indicate that ATAC and NURF regulate overlapping sets of target genes during development. In addition, we find that Ada2a chromosome binding and histone H4-Lys12 acetylation are compromised in Iswi and Nurf301 mutants. Our results strongly suggest that NURF is required for ATAC to access the chromatin and to regulate global chromosome organization.

Référence

EMBO Rep. 2008 Feb;9(2):187-92