Strong ion-exchange centrifugal partition chromatography as an efficient method for the large-scale purification of glucosinolates.

Date publication

novembre 2007

Auteurs

Membres identifiés du Cancéropôle Est :
Dr NUZILLARD Jean-Marc

Tous les auteurs :
Toribio A, Nuzillard JM, Renault JH

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/17904564

Résumé

The glucosinolates sinalbin and glucoraphanin were purified by strong ion-exchange displacement centrifugal partition chromatography (SIXCPC). The optimized conditions involved the biphasic solvent system ethyl acetate/n-butanol/water (3:2:5, v/v), the lipophilic anion-exchanger Aliquat 336 (trioctylmethylammonium chloride, 160 and 408 mM) and a sodium iodide solution (80 and 272 mM) as displacer. Amounts as high as 2.4 g of sinalbin and 2.6g of glucoraphanin were obtained in one step in 2.5 and 3.5h respectively, starting from 12 and 25 g of mustard and broccoli seed aqueous extracts, using a laboratory scale CPC column (200 mL inner volume).

Référence

J Chromatogr A. 2007 Nov 2;1170(1-2):44-51