Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture.

Date publication

janvier 2015

Auteurs

Membres identifiés du Cancéropôle Est :
Pr NOEL Georges

Tous les auteurs :
Burckel H, Josset E, Denis JM, Gueulette J, Slabbert J, Noel G, Bischoff P

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/25371289

Résumé

The present in vitro study aimed to assess the effects of combining the mTOR inhibitor RAD001 and temozolomide (TMZ) together with irradiation by either low-linear energy transfer (LET) radiation (gamma-rays) or high-LET radiation (fast neutrons) on the growth and cell survival of the human glioblastoma cell line U-87. We observed a strong decrease in cell proliferation along with a concomitant increase in cell death as a function of the radiation dose. As expected, high-LET radiation was more effective and induced more sustained damage to DNA than low-LET radiation. While RAD001 in association with TMZ induced autophagic cell death, additional combination with either type of radiation did not further increase its occurrence. On the contrary, apoptosis remained at a low level in all experimental groups.

Référence

Oncol Rep. 2015 Jan;33(1):471-7