Fiche publication
Date publication
janvier 2015
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SCHULTZ Patrick
,
Pr WEISS Etienne
,
Dr ZUBER Guy
Tous les auteurs :
Orlov I, Schertel A, Zuber G, Klaholz B, Drillien R, Weiss E, Schultz P, Spehner D
Lien Pubmed
Résumé
Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focussed Ion Beam slicing coupled to Scanning Electron Microscopy (FIB/SEM) enabled visualization of entire cells with probe localization accuracy in the 10 nm range.
Référence
Sci Rep. 2015 Feb 9;5:8324
