Fiche publication


Date publication

décembre 2006

Auteurs

Membres identifiés du Cancéropôle Est :
Dr JEULIN Hélène


Tous les auteurs :
Jeulin H, Grancher N, Kedzierewicz F, Le Faou AE, Venard V

Résumé

The objective of this work was to study the antiviral activity of ribavirin on measles encephalitis infection when using cyclodextrins as carriers. The use of cyclic oligosaccharides can promote the activity of many drugs and the benefit of the association of ribavirin with beta-cyclodextrin has already been demonstrated in vitro. Intracranial inoculation of the rodent adapted neurovirulent CAM/RB strain of measles virus induces encephalitis in CBA/ca mice. The antiviral activity of the complex ribavirin/beta-cyclodextrinat molar ratio 1:1 has been evaluated in vivo in the above encephalitis model. CBA/ca mice were; treated with daily intraperitoneal injection of ribavirin (40 mg/kg) with or without cyclodextrin. The viral load in the brain of mice was quantified by real-time Reverse transcription-Polymerase chain reaction. Treatment of mice by the complex ribavirin/beta-cyclodextrin (1:1) by intraperitoneal route decreases the viral load in the brain of 1.1 and 0.7 log(10) Eq copies x mL(-1) compared to distillated water and ribavirin treatment, respectively. At the same time, free ribavirin injection shows a negligible difference compared to treatment by distillated water. (c) 2006 Elsevier Masson SAS. All rights reserved.

Référence

Pathol Biol (Paris). 2006 Dec;54(10):541-4