NMR in the SPINE Structural Proteomics project.

Date publication

octobre 2006

Auteurs

Membres identifiés du Cancéropôle Est :
Dr KIEFFER Bruno , Dr TRAVE Gilles

Tous les auteurs :
Ab E, Atkinson AR, Banci L, Bertini I, Ciofi-Baffoni S, Brunner K, Diercks T, Dotsch V, Engelke F, Folkers GE, Griesinger C, Gronwald W, Gunther U, Habeck M, de Jong RN, Kalbitzer HR, Kieffer B, Leeflang BR, Loss S, Luchinat C, Marquardsen T, Moskau D, Neidig KP, Nilges M, Piccioli M, Pierattelli R, Rieping W, Schippmann T, Schwalbe H, Trave G, Trenner J, Wohnert J, Zweckstetter M, Kaptein R

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/17001092

Résumé

This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe (SPINE) consortium. Focusing on the development of high-throughput (HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless (13)C-direct detection methods and inferential structure determinations (ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.

Référence

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1150-61