Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site.

Date publication

juin 2006

Auteurs

Membres identifiés du Cancéropôle Est :
Pr KARCHER Gilles

Tous les auteurs :
Tran N, Poussier S, Franken PR, Maskali F, Groubatch F, Vanhove C, Antunes L, Karcher G, Villemot JP, Marie PY

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/16572303

Résumé

PURPOSE: Cell therapy using bone marrow mesenchymal stem cells (BMSCs) shows promise in the treatment of myocardial infarction (MI) but accurate cell delivery within MI areas remains critical. In the present study, we tested the feasibility of in vivo pinhole SPECT imaging for monitoring the sites of intramyocardial implanted BMSCs in relation to targeted MI areas in rats. METHODS: BMSCs were labelled with (111)In-oxine and injected within the fibrotic areas of 3-month-old MI in ten rats. Two days later, dual (111)In/(99m)Tc-sestamibi pinhole SPECT was recorded for localisation of (111)In-BMSCs on a 15-segment left ventricular (LV) division. Additional (99m)Tc-sestamibi pinhole SPECT had been performed 1 month earlier and on the day before transplantation. In vitro counting on histological sections was used to validate the pinhole SPECT determination of (111)In-BMSC activity within LV segments. RESULTS: The underperfused MI area (segments with

Référence

Eur J Nucl Med Mol Imaging. 2006 Jun;33(6):709-15