Fiche publication
Date publication
janvier 2006
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ROYER Bernard
Tous les auteurs :
Davani S, Vergely C, Royer B, Bouhaddi M, Reyssie H, Rochette L, Kantelip J
Résumé
Previous studies indicate that adenosine supplementation or nitric oxide synthase ( NOS) inhibition during reperfusion exert protective effects against myocardial ischemia-reperfusion ( I/R) injury. We wanted to test the hypothesis that NOS inhibition before I/R also protects the myocardium against further injury and aimed to determine the involvement of adenosine receptors in a perfused rat heart model. Rats were injected with 10mg/kg of L-NAME ( N-omega-nitro-(L)-arginine methyl ester) or L-NAME + SPT ( 8-( p-sulfophenyl)-theophylline) - an adenosine antagonist - at 2 x 25mg/kg or with a saline buffer, 24hrs prior to heart excision. The hearts, perfused retrogradely were subjected to 60min of global ischemia followed by 120min reperfusion. L-NAME decreased NOx ( nitrite and nitrate) production ( 16.2 +/- 3.2 vs. 7.0 +/- 1.8 mu mol/L; P
Référence
Cell Mol Biol. 2006;52 Suppl. S:868-73.
