Fiche publication
Date publication
novembre 2005
Auteurs
Membres identifiés du Cancéropôle Est :
Pr FROMM Michel
Tous les auteurs :
Gaillard S, Ross CJ, Armbruster V, Hill MA, Stevens DL, Gharbi T, Fromm M
Résumé
In radiobiology, low doses of high-LET radiation correspond to a few particle traversals through the cell population. Therefore, for studies on cell monolayers irradiated with a low dose of alpha-particles, it is extremely useful if the number and position of particle traversals can be determined. In this study we describe a new method, based on UV-curing, to obtain a 10 mu m thick CR-39 grafted onto a 2.5 mu m thick PolyEthylene Terephtalate (PET). This thin double polymeric layer, used as a dish base, has a regular and reproducible detector thickness which can be traversed by 3.5 MeV alpha-particles, with a sufficient residual energy to traverse mammalian cells attached to the base. The recording properties of a PET-CR-39 dish, together with a demonstration of its use for radiobiological experiments, are presented. This new tool allows the precise determination of single-track impact parameters at a sub-cellular level. (c) 2005 Elsevier Ltd. All rights reserved.
Référence
Radiat Meas. 2005 Nov;40(2-6):279-82.
