Fiche publication
Date publication
octobre 2005
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BAGUET Aurélie
,
Dr TOMASETTO Catherine
Tous les auteurs :
Ballut L, Marchadier B, Baguet A, Tomasetto C, Seraphin B, Le Hir H
Lien Pubmed
Résumé
The multiprotein exon junction complex (EJC) is assembled on mRNAs as a consequence of splicing. EJC core components maintain a stable grip on mRNAs even as the overall EJC protein composition evolves while mRNAs travel to the cytoplasm. Here we show that recombinant EJC subunits MLN51, MAGOH and Y14, together with the DEAD-box protein eIF4AIII bound to ATP, are necessary and sufficient to form a highly stable complex on single-stranded RNA. Cross-linking and RNase protection studies indicate that this recombinant complex recapitulates the EJC core. The stable association of the recombinant EJC core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. We elucidate the modalities of EJC binding to RNA and provide the first example of how cellular machineries may use RNA helicases to clamp several proteins onto RNA in stable and sequence-independent manners.
Référence
Nat Struct Mol Biol. 2005 Oct;12(10):861-9
