MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles.

Date publication

juin 2004

Auteurs

Membres identifiés du Cancéropôle Est :
Pr LAQUERRIERE Patrice

Tous les auteurs :
Laquerriere P, Grandjean-Laquerriere A, Addadi-Rebbah S, Jallot E, Laurent-Maquin D, Frayssinet P, Guenounou M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/14751736

Résumé

After calcium-phosphates biomaterials based implantation like hydroxyapatite (HA) coating, particles are released in the periprosthetic tissues. Wear-debris induced fibrous membranes contain macrophage subsets that can produce metalloproteinases (MMPs), which are considered to be key enzymes in extra-cellular matrix turnover. Tissue inhibitors of metalloproteinases (TIMPs) are important regulator of MMPs activity. Interleukin-1 mainly produced by monocytes can also regulate MMPs production. In the present work, we have evaluated the effect of HA particles characteristics (size, shape and sintering temperature) on the MMP-2, -9 and their respective inhibitors TIMP-2, -1 production. Our results demonstrate that sintering temperature (that modify crystal size and surface area) have little effect on MMPs and TIMPs production. Non-phagocytable particles induced more MMP-9, although phagocytable particles induced more IL-1beta release. The shape of the particles was the most important factor since needle-shaped particles induced the most significant up-regulated expression of MMPs and IL-1beta.

Référence

Biomaterials. 2004 Jun;25(13):2515-24.