Identification of hTAF(II)80 delta links apoptotic signaling pathways to transcription factor TFIID function.

Date publication

septembre 2001

Auteurs

Membres identifiés du Cancéropôle Est :
Dr TORA Laszlo

Tous les auteurs :
Bell B, Scheer E, Tora L

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/11583621

Résumé

Apoptotic cell death is associated with altered levels of mRNA expression, yet the mechanisms that coordinate changes in gene expression with activation of the cell death machinery remain obscure. Here, we report the cloning and characterization of hTAF(II)80 delta, a specialized isoform of the general transcription factor TFIID subunit hTAF(II)80. Several distinct apoptotic stimuli induce the expression and caspase-dependent cleavage of hTAF(II)80 delta. hTAF(II)80 delta, unlike hTAF(II)80, forms a TFIID-like complex lacking hTAF(II)31. Elevated expression of hTAF(II)80 delta in HeLa cells is sufficient to trigger apoptotic cell death and selectively alters cellular transcription, including the induction of the target genes gadd45 and p21. These data define a signaling pathway that couples apoptotic signals to a reprogramming of RNA polymerase II transcription.

Référence

Mol Cell. 2001 Sep;8(3):591-600.