Fiche publication
Date publication
janvier 2017
Journal
PloS one
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DELMAS Dominique
,
Pr GHIRINGHELLI François
,
Dr RIALLAND Mickaël
,
Dr REBE Cédric
,
Pr KAHN Naim
,
Dr HICHAMI Aziz
Tous les auteurs :
Zeriouh W, Nani A, Belarbi M, Dumont A, de Rosny C, Aboura I, Ghanemi FZ, Murtaza B, Patoli D, Thomas C, Apetoh L, Rébé C, Delmas D, Akhtar Khan N, Ghiringhelli F, Rialland M, Hichami A
Lien Pubmed
Résumé
Dietary polyphenols, derived from natural products, have received a great interest for their chemopreventive properties against cancer. In this study, we investigated the effects of phenolic extract of the oleaster leaves (PEOL) on tumor growth in mouse model and on cell death in colon cancer cell lines. We assessed the effect of oleaster leaf infusion on HCT116 (human colon cancer cell line) xenograft growth in athymic nude mice. We observed that oleaster leaf polyphenol-rich infusion limited HCT116 tumor growth in vivo. Investigations of PEOL on two human CRC cell lines showed that PEOL induced apoptosis in HCT116 and HCT8 cells. We demonstrated an activation of caspase-3, -7 and -9 by PEOL and that pre-treatment with the pan-caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (z-VAD-fmk), prevented PEOL-induced cell death. We observed an involvement of the mitochondrial pathway in PEOL-induced apoptosis evidenced by reactive oxygen species (ROS) production, a decrease of mitochondrial membrane potential, and cytochrome c release. Increase in intracellular Ca2+ concentration induced by PEOL represents the early event involved in mitochondrial dysfunction, ROS-induced endoplasmic reticulum (ER) stress and apoptosis induced by PEOL, as ruthenium red, an inhibitor of mitochondrial calcium uptake inhibited apoptotic effect of PEOL, BAPTA/AM inhibited PEOL-induced ROS generation and finally, N-acetyl-L-cysteine reversed ER stress and apoptotic effect of PEOL. These results demonstrate that polyphenols from oleaster leaves might have a strong potential as chemopreventive agent in colorectal cancer.
Mots clés
Animals, Apoptosis, drug effects, Caspases, metabolism, Cell Death, drug effects, Cell Line, Tumor, Cell Proliferation, drug effects, Colonic Neoplasms, pathology, Endoplasmic Reticulum Stress, drug effects, Gene Expression Regulation, Neoplastic, drug effects, Humans, Membrane Potential, Mitochondrial, drug effects, Mice, Mice, Nude, Mitochondria, drug effects, Olea, chemistry, Phenol, chemistry, Plant Extracts, pharmacology, Plant Leaves, chemistry, Reactive Oxygen Species, metabolism, Transcription Factor CHOP, metabolism, Xenograft Model Antitumor Assays
Référence
PLoS ONE. 2017 ;12(2):e0170823
