Fiche publication
Date publication
août 2006
Journal
Archives of virology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CLAVEL Christine
Tous les auteurs :
Fleury MJ, Touzé A, Alvarez E, Carpentier G, Clavel C, Vautherot JF, Coursaget P
Lien Pubmed
Résumé
The majority of the neutralizing epitopes of papillomaviruses (PV) are conformation-specific and have not been fully characterised. Studies have, to date, been limited to a few HPV types only. We analysed the epitopes on the major capsid protein (L1) of Human papillomavirus (HPV) type 31 using monoclonal antibodies (MAbs) generated against HPV-31 virus-like particles (VLPs). The type-specific MAbs against HPV-31 were all found to be neutralizing and recognized conformation-dependent epitopes. Two other MAbs directed against a conformational epitope were found to be cross-reactive with other HPV types, and one of them was found to be cross-neutralizing. Cross-reactive antibodies were further investigated using wild-type HPV-16 L1 VLPs and two mutants. The results obtained suggested the existence of a cross-neutralizing conformational epitope at the N-terminal part of the FG loop of the major capsid protein, and the other four cross-reactive MAbs recognized epitopes also located at the N-terminal part of the FG loop.
Mots clés
Amino Acid Sequence, Animals, Antibodies, Monoclonal, blood, Capsid Proteins, chemistry, Cells, Cultured, Cross Reactions, Epitope Mapping, Epitopes, immunology, Female, Humans, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Neutralization Tests, Oncogene Proteins, Viral, chemistry, Papillomaviridae, classification, Peptides, chemistry, Protein Conformation, Spodoptera, Virion, immunology
Référence
Arch. Virol.. 2006 Aug;151(8):1511-23
