Fiche publication
Date publication
janvier 1991
Journal
European journal of cancer (Oxford, England : 1990)
Lien Pubmed
Résumé
The optimisation of the formulation of thermosensitive, doxorubicin-containing small unilamellar liposomes is described. The liposomes were first strictly defined in terms of size distribution and size stability and a quality level was defined. The suspension contained more than 95% vesicles with a maximal diameter of 50 nm and kept this level for a minimum of 24 hours. Several lipid mixtures were tested in defined thermal conditions usable for in vitro experiments: 43 degrees C in fetal calf serum-containing medium. The mixture yielding the best differential thermal stability (DTS) defined as the difference of release between 37 degrees C and 43 degrees C exposures was found to be a dipalmitoylphosphatidylcholine/distearoylphosphatidyl-choline/chol esterol mixture in 5:4:2 molar ratio yielding 72% DTS. These thermosensitive liposomes were evaluated between pH 6.00 and 8.00 since hyperthermia-induced lethality was reported to be enhanced by pH variations. Their release capacity was not altered by any pH variations. Incorporation of doxorubicin within these liposomes was then performed. The release kinetics at 37 degrees and 43 degrees C were determined. It is proposed to use this formulation in in vitro experiments on tumour cells, although a decrease of DTS was evident.
Mots clés
Doxorubicin, administration & dosage, Drug Carriers, Drug Compounding, methods, Drug Stability, Hot Temperature, Hydrogen-Ion Concentration, Kinetics, Liposomes
Référence
Eur. J. Cancer. 1991 ;27(8):1026-30
