Fiche publication
Date publication
décembre 2001
Journal
The European journal of neuroscience
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHAMBON Pierre
,
Dr METZGER Daniel
Tous les auteurs :
Weber P, Metzger D, Chambon P
Lien Pubmed
Résumé
To develop spatio-temporally controlled somatic mutagenesis in the adult mouse nervous system, we established transgenic mice expressing the tamoxifen-inducible Cre-ERT recombinase under the control of the mouse prion protein (PrP) promoter. Cre-ERT was expressed in most regions of the brain and in the retina of one transgenic line, whereas its expression was mostly restricted to the hippocampus and the cerebellum in another line. As tamoxifen efficiently induced Cre-mediated recombination in the various neuronal cell types expressing Cre-ERT in the brain of adult mice, the PrP-Cre-ERT lines should be valuable tools to study the functions of genes involved in neurodegenerative diseases or regeneration, and in complex processes such as behaviour, learning and memory. Some limitations of presently available reporter lines for Cre-mediated recombination in adult mouse CNS are discussed.
Mots clés
Animals, Brain, embryology, Female, Genes, Reporter, genetics, Immunohistochemistry, Integrases, genetics, Male, Mice, Mice, Transgenic, genetics, Mutagenesis, genetics, Neurons, metabolism, PrPC Proteins, genetics, Promoter Regions, Genetic, genetics, Viral Proteins, genetics, beta-Galactosidase, genetics
Référence
Eur. J. Neurosci.. 2001 Dec;14(11):1777-83
