Bio-specific and bio-orthogonal chemistries to switch-off the quencher of a FRET-based fluorescent probe: application to living-cell biothiol imaging.

Date publication

septembre 2014

Auteurs

Membres identifiés du Cancéropôle Est :
Dr WAGNER Alain

Tous les auteurs :
Egloff C, Jacques SA, Nothisen M, Weltin D, Calligaro C, Mosser M, Remy JS, Wagner A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/25034617

Résumé

We report the first molecular system that is responsive to both a bio-specific and a bio-orthogonal stimulus. This dual activation process was applied to the design of a biothiol-specific FRET-based fluorescent probe that could be turned-on via an original concept of quencher bleaching.

Référence

Chem Commun (Camb). 2014 Sep 11;50(70):10049-51