Fiche publication
Date publication
décembre 2000
Journal
Journal of photochemistry and photobiology. B, Biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BEZDETNAYA-BOLOTINE Lina
,
Dr VIGNERON Céline
Tous les auteurs :
Cosserat-Gerardin I, Bezdetnaya L, Notter D, Vigneron C, Guillemin F
Lien Pubmed
Résumé
Hexylester of 5-aminolevulinic acid (hALA) has been considered as an alternative to 5-aminolevulinic acid (ALA) for the treatment of malignancies of different origin. The present study addresses the ALA and hALA-induced PpIX pharmacokinetic profile using rat bladder carcinoma cells (AY27). The total PpIX content measured spectrofluorimetrically after extraction procedure at the end of 2 h incubation was at least 1.5-fold greater with hALA compared to ALA despite the difference in concentration of several orders between the two compounds (1 or 5 mM ALA and 5 or 10 x 10(-3) mM hALA). Considerable PpIX efflux was detected in the extracellular medium at the end of the incubation. With 5 mM ALA and 10 x 10(-3) mM hALA, PpIX build-up was continued beyond the incubation period pointing out to enzyme saturation in the biosynthetic pathway or/and the constitution of ALA reserve. Red laser light (lambda=630 nm) irradiation of AY27 cells after 2 h incubation with increasing ALA or hALA concentrations resulted in a nearly equal photocytotoxicity.
Mots clés
Aminolevulinic Acid, pharmacology, Animals, Cell Extracts, Cell Survival, Culture Media, Culture Media, Serum-Free, Dose-Response Relationship, Drug, Fluorescence, Photochemotherapy, methods, Photosensitizing Agents, pharmacokinetics, Protoporphyrins, biosynthesis, Rats, Tumor Cells, Cultured, Urinary Bladder Neoplasms, drug therapy
Référence
J. Photochem. Photobiol. B, Biol.. 2000 Dec;59(1-3):72-9
