Fiche publication
Date publication
juillet 2018
Journal
Toxicology and applied pharmacology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DUBOIS-POT-SCHNEIDER Hélène
,
Pr RIHN Bertrand
Tous les auteurs :
Chézeau L, Sébillaud S, Safar R, Seidel C, Dembélé D, Lorcin M, Langlais C, Grossmann S, Nunge H, Michaux S, Schneider HDP, Rihn B, Joubert O, Binet S, Cosnier F, Gaté L
Lien Pubmed
Résumé
The number of workers potentially exposed to nanoparticles (NPs) during industrial processes is increasing, although the toxicological properties of these compounds still need to be fully characterized. As NPs may be aerosolized during industrial processes, inhalation represents their main route of occupational exposure. Here, the short- and long-term pulmonary toxicological properties of titanium dioxide were studied, using conventional and molecular toxicological approaches. Fischer 344 rats were exposed to 10 mg/m of a TiO nanostructured aerosol (NSA) by nose-only inhalation for 6 h/day, 5 days/week over 4 weeks. Lung samples were collected up to 180 post-exposure days. Biochemical and cytological analyses of bronchoalveolar lavage (BAL) showed a strong inflammatory response up to 3 post-exposure days, which decreased overtime. In addition, gene expression profiling revealed overexpression of genes involved in inflammation that was maintained 6 months after the end of exposure (long-term response). Genes involved in oxidative stress and vascular changes were also up-regulated. Long-term response was characterized by persistent altered expression of a number of genes up to 180 post-exposure days, despite the absence of significant histopathological changes. The physiopathological consequences of these changes are not fully understood, but they should raise concerns about the long-term pulmonary effects of inhaled biopersistent NPs such as TiO.
Mots clés
Gene expression profile, Lung inflammation, Short- and long-term response, Titanium dioxide
Référence
Toxicol. Appl. Pharmacol.. 2018 Jul 13;:
