Fiche publication
Date publication
juillet 2018
Journal
Chemical science
Auteurs
Membres identifiés du Cancéropôle Est :
Dr KIEFFER Bruno
Tous les auteurs :
Boehringer R, Kieffer B, Torbeev V
Lien Pubmed
Résumé
Discovering molecular probes that specifically recognize distinct amyloid structures is highly important for physiological studies of protein-misfolding diseases as well as for the development of diagnostic reagents and inhibitors of amyloid self-assembly. Here, we demonstrate an approach that allows for identification of -methylated peptides that are specific binders for a particular amyloid fiber subtype (or polymorph). Protein design and chemical synthesis were used to produce covalently tethered amyloid analogues with molecular masses approaching 24 kDa and containing nine copies of an amyloidogenic peptide. Such engineered constructs served as a molecular testing platform to evaluate the aggregation properties and solubility as a function of -methylation pattern. An advantage of the method is the possibility of biophysical characterization of amyloid constructs in solution.
Référence
Chem Sci. 2018 Jul 7;9(25):5594-5599
