Fiche publication
Date publication
mai 2018
Journal
Journal of biophotonics
Auteurs
Membres identifiés du Cancéropôle Est :
Pr PIOT Olivier
Tous les auteurs :
Sebiskveradze D, Bertino B, Gaydou V, Dugaret AS, Roquet M, Zugaj DE, Voegel JJ, Jeannesson P, Manfait M, Piot O
Lien Pubmed
Résumé
Skin is one of the most important organs of the human body because of its characteristics and functions. There are many alterations, either pathological or physiological, that can disturb its functioning. However, at present all methods used to investigate skin diseases, non-invasive or invasive, are based on clinical examinations by physicians. Thus, diagnosis, prognosis and therapeutic management rely on the expertise of the practitioner, the quality of the method and the accessibility of distinctive morphological characteristics of each lesion. To overcome the high sensitivity of these parameters, techniques based on more objective criteria must be explored. Vibrational spectroscopy has become as a key technique for tissue analysis in the biomedical research field. Based on a non-destructive light/matter interaction, this tool provides information about specific molecular structure and composition of the analyzed sample, thus relating to its precise physiopathological state and permitting to distinguish lesional from normal tissues. This label-free optical method can be performed directly on the paraffin embedded tissue sections without chemical de-waxing. In this study, the potential of the infrared micro-spectroscopy, combined with data classification methods was demonstrated, to characterize at the tissular level different types of inflammatory skin lesions, and this independently from conventional histopathology.
Mots clés
IR spectral microimaging, predictive classification, quantitative spectral histopathology, skin inflammation, unsupervised clustering
Référence
J Biophotonics. 2018 May 1;:e201700380
