Fiche publication
Date publication
juillet 2008
Journal
Sensors (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOIREAU Wilfrid
,
Pr DELAGE-MOURROUX Régis
,
Dr ELIE-CAILLE Céline
Tous les auteurs :
Berthier A, Elie-Caille C, Lesniewska E, Delage-Mourroux R, Boireau W
Lien Pubmed
Résumé
We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA.
Mots clés
AFM, Nano-objects, Protein/DNA interaction., SPR, molecular lego
Référence
Sensors (Basel). 2008 Jul;8(7):4413-4428
