Fiche publication
Date publication
décembre 2004
Journal
Biochemical and biophysical research communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOIREAU Wilfrid
,
Pr DELAGE-MOURROUX Régis
,
Dr FRAICHARD Annick
Tous les auteurs :
Mansuy V, Boireau W, Fraichard A, Schlick JL, Jouvenot M, Delage-Mourroux R
Lien Pubmed
Résumé
We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA(A) receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA(A) receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA(A) receptor.
Mots clés
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, CHO Cells, Cricetinae, Dose-Response Relationship, Drug, Escherichia coli, genetics, Gene Expression, Glutathione Transferase, metabolism, Green Fluorescent Proteins, genetics, Humans, Intracellular Space, metabolism, Microtubule-Associated Proteins, chemistry, Microtubules, metabolism, Molecular Sequence Data, Protein Binding, Protein Structure, Tertiary, Protein Subunits, Receptors, GABA-A, chemistry, Recombinant Fusion Proteins, chemistry, Sequence Alignment, Tubulin, chemistry
Référence
Biochem. Biophys. Res. Commun.. 2004 Dec;325(2):639-48
