Two new bis-iridoids isolated from Scabiosa stellata and their antibacterial, antioxidant, anti-tyrosinase and cytotoxic activities.

Fiche publication

Date publication

mars 2018

Journal

Fitoterapia

Auteurs

Membres identifiés du Cancéropôle Est :
Pr MORJANI Hamid , Dr NUZILLARD Jean-Marc

Tous les auteurs :
Lehbili M, Alabdul Magid A, Hubert J, Kabouche A, Voutquenne-Nazabadioko L, Renault JH, Nuzillard JM, Morjani H, Abedini A, Gangloff SC, Kabouche Z

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/29273413

Résumé

This study presents the chemical profile investigation of a 70% ethanol extract obtained from Scabiosa stellata, a medicinal herbaceous traditionally used to treat heel cracks. A C NMR-based dereplication methodology was firstly applied on centrifugal partition chromatography-generated fractions in order to quickly identify the major compounds of the extract. The dereplication process was then completed by semi-preparative high-performance liquid chromatography in order to identify unknown or minor compounds. Two new bis-iridoids, namely 7-O-caffeoyl-sylvestroside I (1) and 7-O-(p-coumaroyl)-sylvestroside I (2), together with ten known compounds (3-12) were isolated. Their structures were elucidated by spectroscopic methods including NMR and HR-ESI-MS. The antibacterial, anti-tyrosinase and DPPH radical scavenging activities of the crude extract, fractions, and isolated compounds were evaluated. A significant antibacterial activity was observed for nine isolated compounds, particularly 1 and 2 which yielded MIC values of 31.2μg/mL against Enterococcus faecalis and 62.5μg/mL against Staphylococcus epidermidis. The cytotoxic activity of these new bis-iridoids was evaluated on a fibrosarcoma cell line (HT1080) and only compound 1 exhibited a moderate cytotoxic activity (IC 35.9μg/mL).