FRET-SLiM on native autofluorescence: a fast and reliable method to study interactions between fluorescent probes and lignin in plant cell wall.

Date publication

janvier 2018

Journal

Plant methods

Auteurs

Membres identifiés du Cancéropôle Est :
Dr TERRYN Christine

Tous les auteurs :
Terryn C, Paës G, Spriet C

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/30154910

Résumé

Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that necessitates some pretreatments and several types of catalysts to be transformed efficiently. In particular, enzymes degrading lignocellulose can become inactivated due to their binding to lignin through non-specific interactions, leading to a loss in catalytic efficiency of industrial processes. Gaining more knowledge in the strength of interactions would allow optimizing enzymes and selecting appropriate pretreatments.

Mots clés

Autofluorescence, FRET, Fluorescence lifetime, Interaction, Lignocellulose, SLiM

Référence

Plant Methods. 2018 ;14:74