Fiche publication
Date publication
janvier 2018
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Mme MESSADDEQ Nadia
Tous les auteurs :
Yefimova MG, Messaddeq N, Meunier AC, Cantereau A, Jegou B, Bourmeyster N
Lien Pubmed
Résumé
Sertoli cells were discovered in the seminiferous tubules by Enrico Sertoli in 1865 (Morgagni 7:31-33, 1865). Intense phagocytosis is, in the context of spermatogenesis cycle, morphologically the most noticeable function of Sertoli cells. In this chapter the major principles of phagocytosis machinery and its specificities in the seminiferous tubules will be briefly reviewed, guidelines of analysis of main phagocytosis steps by confocal and transmission electron microscopy will be described, and a simplified method to assess phagocytosis rate in routine experiments will be given.
Mots clés
Apoptotic-like membranes, Homeostatic phagocytosis, Nonprofessional phagocytes, Phagocytosis steps, Residual bodies, Sertoli cells
Référence
Methods Mol. Biol.. 2018 ;1748:85-101
