Orthogonal Operation of Constitutional Dynamic Networks Consisting of DNA-Tweezer Machines.

Fiche publication

Date publication

décembre 2017

Journal

ACS nano

Auteurs

Membres identifiés du Cancéropôle Est :
Pr LEHN Jean-Marie

Tous les auteurs :
Yue L, Wang S, Cecconello A, Lehn JM, Willner I

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/29140681

Résumé

Overexpression or down-regulation of cellular processes are often controlled by dynamic chemical networks. Bioinspired by nature, we introduce constitutional dynamic networks (CDNs) as systems that emulate the principle of the nature processes. The CDNs comprise dynamically interconvertible equilibrated constituents that respond to external triggers by adapting the composition of the dynamic mixture to the energetic stabilization of the constituents. We introduce a nucleic acid-based CDN that includes four interconvertible and mechanically triggered tweezers, AA', BB', AB' and BA', existing in closed, closed, open, and open configurations, respectively. By subjecting the CDN to auxiliary triggers, the guided stabilization of one of the network constituents dictates the dynamic reconfiguration of the structures of the tweezers constituents. The orthogonal and reversible operations of the CDN DNA tweezers are demonstrated, using T-A·T triplex or K-stabilized G-quadruplex as structural motifs that control the stabilities of the constituents. The implications of the study rest on the possible applications of input-guided CDN assemblies for sensing, logic gate operations, and programmed activation of molecular machines.