Fiche publication
Date publication
novembre 2017
Journal
Molecular biology of the cell
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOETZ Jacky
,
Dr HARLEPP Sébastien
Tous les auteurs :
Harlepp S, Thalmann F, Follain G, Goetz JG
Lien Pubmed
Résumé
Force sensing and generation at the tissue and cellular scale is central to many biological events. There is a growing interest in modern cell biology for methods enabling force measurements in vivo. Optical trapping allows noninvasive probing of piconewton forces and thus emerged as a promising mean for assessing biomechanics in vivo. Nevertheless, the main obstacles lie in the accurate determination of the trap stiffness in heterogeneous living organisms, at any position where the trap is used. A proper calibration of the trap stiffness is thus required for performing accurate and reliable force measurements in vivo. Here we introduce a method that overcomes these difficulties by accurately measuring hemodynamic profiles in order to calibrate the trap stiffness. Doing so, and using numerical methods to assess the accuracy of the experimental data, we measured flow profiles and drag forces imposed to trapped red blood cells of living zebrafish embryos. Using treatments enabling blood flow tuning, we demonstrated that such a method is powerful in measuring hemodynamic forces in vivo with accuracy and confidence. Altogether this study demonstrates the power of optical tweezing in measuring low range hemodynamic forces in vivo and offers an unprecedented tool in both cell and developmental biology.
Mots clés
Biomechanical Phenomena, physiology, Calibration, Equipment and Supplies, Hemodynamics, physiology, Mechanical Phenomena, Optical Tweezers, Physical Phenomena, Research Design, Stress, Mechanical
Référence
Mol. Biol. Cell. 2017 Nov 7;28(23):3252-3260
