Fiche publication
Date publication
septembre 2017
Journal
Development (Cambridge, England)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr TORA Laszlo
Tous les auteurs :
Bardot P, Vincent SD, Fournier M, Hubaud A, Joint M, Tora L, Pourquié O
Lien Pubmed
Résumé
During development, tightly regulated gene expression programs control cell fate and patterning. A key regulatory step in eukaryotic transcription is the assembly of the pre-initiation complex (PIC) at promoters. The PIC assembly has mainly been studied in vitro, and little is known about its composition during development. In vitro data suggests that TFIID is the general transcription factor that nucleates PIC formation at promoters. Here we show that TAF10, a subunit of TFIID and of the transcriptional co-activator SAGA, is required for the assembly of these complexes in the mouse embryo. We performed Taf10 conditional deletions during mesoderm development and show that Taf10 loss in the presomitic mesoderm (PSM) does not prevent cyclic gene transcription or PSM segmental patterning, while lateral plate differentiation is profoundly altered. During this period, global mRNA levels are unchanged in the PSM, with only a minor subset of genes dysregulated. Together, our data strongly suggest that the TAF10-containing canonical TFIID and SAGA complexes, are dispensable for early paraxial mesoderm development, arguing against the generic role in transcription proposed for these fully assembled holo complexes.
Mots clés
Conditional knockout, Mouse, Paraxial mesoderm, Presomitic mesoderm, Proteomic, RNA polymerase II, TATA binding protein
Référence
Development. 2017 Sep;:
