Fiche publication
Date publication
septembre 2017
Journal
Molecular cell
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DEVYS Didier
,
Dr TORA Laszlo
Tous les auteurs :
Warfield L, Ramachandran S, Baptista T, Devys D, Tora L, Hahn S
Lien Pubmed
Résumé
Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly dependent on TFIID function. Degron-dependent depletion of Taf1, Taf2, Taf7, Taf11, and Taf13 showed similar transcription decreases for genes in the Taf1-depleted, Taf1-enriched, TATA-containing, and TATA-less gene classes. The magnitude of TFIID dependence varies with growth conditions, although this variation is similar genome-wide. Many studies have suggested differences in gene-regulatory mechanisms between TATA and TATA-less genes, and these differences have been attributed in part to differential dependence on SAGA or TFIID. Our work indicates that TFIID participates in expression of nearly all yeast mRNAs and that differences in regulation between these two gene categories is due to other properties.
Mots clés
Gene Deletion, Gene Expression Regulation, Fungal, Genome, Fungal, Promoter Regions, Genetic, Protein Subunits, genetics, RNA Polymerase II, genetics, RNA, Fungal, genetics, RNA, Messenger, genetics, Saccharomyces cerevisiae, genetics, Saccharomyces cerevisiae Proteins, chemistry, TATA-Binding Protein Associated Factors, deficiency, TATA-Box Binding Protein, genetics, Trans-Activators, chemistry, Transcription Factor TFIID, deficiency, Transcription, Genetic
Référence
Mol. Cell. 2017 Sep;:
