Raman spectroscopy-based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes.

Fiche publication

Date publication

décembre 2018

Journal

Journal of biophotonics

Auteurs

Membres identifiés du Cancéropôle Est :
Pr SOCKALINGUM Ganesh

Tous les auteurs :
Szafraniec E, Kus E, Wislocka A, Kukla B, Sierka E, Untereiner V, Sockalingum GD, Chlopicki S, Baranska M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/30578586

Résumé

Liver sinusoidal endothelial cells (LSECs), a type of endothelial cells with unique morphology and function, play an important role in the liver hemostasis and LSECs dysfunction is involved in the development of non-alcoholic fatty liver disease (NAFLD). Here, we employed Raman imaging and chemometric data analysis in order to characterize the presence of lipid droplets (LDs) and their lipid content in primary murine LSECs, in comparison with hepatocytes, isolated from mice on high fat diet. Upon NAFLD development, LDs content in LSECs changed towards more unsaturated lipids and this response was associated with an increased expression of SCD-1. To the best of our knowledge, this is a first report characterizing LDs in LSECs, where their chemical composition is analyzed along the progression of NAFLD at the level of single LD using Raman imaging. This article is protected by copyright. All rights reserved.