Fiche publication
Date publication
janvier 2019
Journal
Journal of applied toxicology : JAT
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GRANDEMANGE Stéphanie
,
Pr RIHN Bertrand
Tous les auteurs :
Nahle S, Safar R, Grandemange S, Foliguet B, Lovera-Leroux M, Doumandji Z, Le Faou A, Joubert O, Rihn B, Ferrari L
Lien Pubmed
Résumé
Human exposure to airborne carbon nanotubes (CNT) is increasing because of their applications in different sectors; therefore, they constitute a biological hazard. Consequently, developing studies on CNT toxicity become a necessity. CNTs can have different properties in term of length, size and charge. Here, we compared the cellular effect of multiwall (MWCNTs) and single wall CNTs (SWCNTs). MWCNTs consist of multiple layers of graphene, while SWCNTs are monolayers. The effects of MWCNTs and SWCNTs were evaluated by the water-soluble tetrazolium salt cell proliferation assay on NR8383 cells, rat alveolar macrophage cell line (NR8383). After 24 hours of exposure, MWCNTs showed higher toxicity (50% inhibitory concentration [IC ] = 3.2 cm /cm ) than SWCNTs (IC = 44 cm /cm ). Only SWCNTs have induced NR8383 cells apoptosis as assayed by flow cytometry using the annexin V/IP staining test. The expression of genes involved in oxidative burst (Ncf1), inflammation (Nfκb, Tnf-α, Il-6 and Il-1β), mitochondrial damage (Opa) and apoptotic balance (Pdcd4, Bcl-2 and Casp-8) was determined. We found that MWCNT exposure predominantly induce inflammation, while SWCNTs induce apoptosis and impaired mitochondrial function. Our results clearly suggest that MWCNTs are ideal candidates for acute inflammation induction. In vivo studies are required to confirm this hypothesis. However, we conclude that toxicity of CNTs is dependent on their physical and chemical characteristics.
Mots clés
MWCNT, SWCNT, apoptosis, in vitro, inflammation, multiwall carbon nanotubes, oxidative stress, rat macrophages cell line NR8383, single wall carbon nanotubes
Référence
J Appl Toxicol. 2019 Jan 3;:
