Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry.

Fiche publication

Date publication

juin 2017

Journal

Journal of ovarian research

Auteurs

Membres identifiés du Cancéropôle Est :
Dr AMIOT Clotilde , Pr ROUX Christophe

Tous les auteurs :
Zver T, Mouloungui E, Berdin A, Roux C, Amiot C

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/28645304

Résumé

Ovarian tissue cryopreservation is a technique for fertility preservation addressed to prepubertal girls or to patients for whom no ovarian stimulation is possible before initiation of gonadotoxic treatments. Autotransplantation of frozen-thawed ovarian tissue is the only available option for reuse but presents some limitations: ischemic tissue damages post-transplant and reintroduction of malignant cells in cases of cancer. It is therefore essential to qualify ovarian tissue before autograft on a functional and oncological point of view. Here, we aimed to isolate viable cells from human ovarian cortex in order to obtain an ovarian cell suspension analyzable by multicolor flow cytometry.