Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis.

Fiche publication

Date publication

mars 2017

Journal

The Journal of biological chemistry

Auteurs

Membres identifiés du Cancéropôle Est :
Dr BADER Marie-France , Dr CHASSEROT-GOLAZ Sylvette , Dr HEINTZ Dimitri , Dr VITALE Nicolas

Tous les auteurs :
Kassas N, Tanguy E, Thahouly T, Fouillen L, Heintz D, Chasserot-Golaz S, Bader MF, Grant NJ, Vitale N

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/28115519

Résumé

Phosphatidic acid (PA) is the simplest phospholipid naturally existing in living organisms, but it constitutes only a minor fraction of total cell lipids. PA has attracted considerable attention because it is a phospholipid precursor, a lipid second messenger, and a modulator of membrane shape, and it has thus been proposed to play key cellular functions. The dynamics of PA in cells and in subcellular compartments, however, remains an open question. The recent generation of fluorescent probes for PA, by fusing GFP to PA-binding domains, has provided direct evidence for PA dynamics in different intracellular compartments. Here, three PA sensors were characterized in vitro, and their preferences for different PA species in particular lipidic environments were compared. In addition, the localization of PA in macrophages during frustrated phagocytosis was examined using these PA sensors and was combined with a lipidomic analysis of PA in intracellular compartments. The results indicate that the PA sensors display some preferences for specific PA species, depending on the lipid environment, and the localization study in macrophages revealed the complexity of intracellular PA dynamics.