How to quantify microparticles in RBCs? A validated flow cytometry method allows the detection of an increase in microparticles during storage.

Date publication

mars 2017

Journal

Transfusion

Auteurs

Membres identifiés du Cancéropôle Est :
Pr GARNACHE-OTTOU Francine , Pr SEILLES Estelle , Dr ANGELOT-DELETTRE Fanny

Tous les auteurs :
Gamonet C, Mourey G, Aupet S, Biichle S, Petitjean R, Vidal C, Pugin A, Naegelen C, Tiberghien P, Morel P, Angelot-Delettre F, Seilles E, Saas P, Bardiaux L, Garnache-Ottou F

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/28164307

Résumé

The procoagulant and proinflammatory microparticles (MPs) released during storage of packed red blood cells (pRBCs) can potentially modify transfusion benefits. A robust method to quantify MPs in pRBCs is needed to evaluate their impact in clinical trials.

Mots clés

Blood Preservation, Cell-Derived Microparticles, Cryopreservation, Erythrocytes, Female, Flow Cytometry, methods, Humans, Male, Time Factors

Référence

Transfusion. 2017 Mar;57(3):504-516