NUP98 is rearranged in 3.8% of pediatric AML forming a clinical and molecular homogenous group with a poor prognosis.

Date publication

mars 2017

Journal

Leukemia

Auteurs

Membres identifiés du Cancéropôle Est :
Dr COLLONGE-RAME Marie-Agnès

Tous les auteurs :
Struski S, Lagarde S, Bories P, Puiseux C, Prade N, Cuccuini W, Pages MP, Bidet A, Gervais C, Lafage-Pochitaloff M, Roche-Lestienne C, Barin C, Penther D, Nadal N, Radford-Weiss I, Collonge-Rame MA, Gaillard B, Mugneret F, Lefebvre C, Bart-Delabesse E, Petit A, Leverger G, Broccardo C, Luquet I, Pasquet M, Delabesse E

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/27694926

Résumé

Pediatric acute myeloid leukemia (AML) is a rare disease whose prognosis is highly variable according to factors such as chromosomal abnormalities. Recurrent genomic rearrangements are detected in half of pediatric AML by karyotype. NUcleoPorin 98 (NUP98) gene is rearranged with 31 different fusion partner genes. These rearrangements are frequently undetected by conventional cytogenetics, as the NUP98 gene is located at the end of the chromosome 11 short arm (11p15). By screening a series of 574 pediatric AML, we detected a NUP98 rearrangement in 22 cases (3.8%), a frequency similar to CBFB-MYH11 fusion gene (4.0%). The most frequent NUP98 fusion gene partner is NSD1. These cases are homogeneous regarding their biological and clinical characteristics, and associated with bad prognosis only improved by bone marrow transplantation. We detailed the biological characteristics of these AML by exome sequencing which demonstrated few recurrent mutations (FLT3 ITD, WT1, CEBPA, NBPF14, BCR and ODF1). The analysis of the clonal structure in these cases suggests that the mutation order in the NUP98-rearranged pediatric AML begins with the NUP98 rearrangement leading to epigenetic dysregulations then followed by mutations of critical hematopoietic transcription factors and finally, activation of the FLT3 signaling pathway.

Mots clés

Alleles, Biomarkers, Tumor, CCAAT-Enhancer-Binding Proteins, genetics, Child, Child, Preschool, Epigenesis, Genetic, Exome, Female, Gene Expression Regulation, Leukemic, Gene Frequency, High-Throughput Nucleotide Sequencing, Humans, In Situ Hybridization, Fluorescence, Infant, Infant, Newborn, Leukemia, Myeloid, Acute, diagnosis, Male, Mutation, Nuclear Pore Complex Proteins, genetics, Oncogene Proteins, Fusion, genetics, Prognosis, Signal Transduction, Translocation, Genetic, WT1 Proteins, genetics, fms-Like Tyrosine Kinase 3, metabolism

Référence

Leukemia. 2017 03;31(3):565-572