Fiche publication
Date publication
mars 2017
Journal
FEBS letters
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HAIECH Jacques
Tous les auteurs :
García-Palmero I, Pompas-Veganzones N, Villalobo E, Gioria S, Haiech J, Villalobo A
Lien Pubmed
Résumé
We identified the Grb7 family members, Grb10 and Grb14, as Ca(2+) -dependent CaM-binding proteins using Ca(2+) -dependent CaM-affinity chromatography as we previously did with Grb7. The potential CaM-binding sites were identified and experimentally tested using fluorescent-labeled peptides corresponding to these sites. The apparent affinity constant of these peptides for CaM, and the minimum number of calcium ions bound to CaM that are required for effective binding to these peptides were also determined. We prepared deletion mutants of the three adaptor proteins lacking the identified sites and determined that they lost or strongly diminished their CaM-binding capacity following the sequence Grb7 > > Grb14 > Grb10. More than one CaM-binding site and/or accessory CaM-binding sites appear to exist in Grb10 and Grb14, as compared to a single one present in Grb7.
Mots clés
Grb10, Grb14, Grb7, adaptor proteins, calmodulin, calmodulin-binding sites
Référence
FEBS Lett.. 2017 Mar;:
