Carbon nanotubes as gene carriers: Focus on internalization pathways related to functionalization and properties.

Fiche publication

Date publication

février 2017

Journal

Acta biomaterialia

Auteurs

Membres identifiés du Cancéropôle Est :
Pr DELAGE-MOURROUX Régis , Dr HERVOUET Eric , Pr BOULAHDOUR Hatem

Tous les auteurs :
Caoduro C, Hervouet E, Girard-Thernier C, Gharbi T, Boulahdour H, Delage-Mourroux R, Pudlo M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/27826000

Résumé

Carbon nanotubes represent promising transporters for delivery of DNA and other biomolecules into living cells. Various methods of CNTs surface functionalization have been developed. These are essential to improve CNTs dispersibility and permit their interactions with biological structures that broaden their use in advanced biomedical applications. The present review discusses the different single walled carbon nanotubes and multiwalled carbon nanotubes functionalization methods, leading to the formation of optimized and functionalized-CNT complexes with DNA. F-CNTs are recognized as efficient and promising gene carriers. Emphasis is then placed on the processes used by f-CNTs/DNA complexes to cross cell membranes. Energy independent pathways and uptake mechanisms dependent on energy, such as endocytosis or phagocytosis, are reported by many studies, and if these mechanisms seem contradictory at first sight, a detailed review of the literature illustrates that they are rather complementary. Preferential use of one or the other depends on the DNA and CNTs chemical nature and physical parameters, experimental procedures and cell types.