Fiche publication
Date publication
mars 2019
Journal
European biophysics journal : EBJ
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DELSUC Marc-André
Tous les auteurs :
van Agthoven MA, Lam YPY, O'Connor PB, Rolando C, Delsuc MA
Lien Pubmed
Résumé
Fourier transform ion cyclotron resonance mass analysers (FT-ICR MS) can offer the highest resolutions and mass accuracies in mass spectrometry. Mass spectra acquired in an FT-ICR MS can yield accurate elemental compositions of all compounds in a complex sample. Fragmentation caused by ion-neutral, ion-electron, or ion-photon interactions leads to more detailed structural information on compounds. The most often used method to correlate compounds and their fragment ions is to isolate the precursor ions from the sample before fragmentation. Two-dimensional mass spectrometry (2D MS) offers a method to correlate precursor and fragment ions without requiring precursor isolation. 2D MS therefore enables easy access to the fragmentation patterns of all compounds from complex samples. In this article, the principles of FT-ICR MS are reviewed and the 2D MS experiment is explained. Data processing for 2D MS is detailed, and the interpretation of 2D mass spectra is described.
Mots clés
Fourier transform, Fourier transform ion cyclotron resonance mass spectrometry, Mass spectrometry, Tandem mass spectrometry, Two dimensional
Référence
Eur. Biophys. J.. 2019 Mar 13;:
