Fiche publication
Date publication
mai 2019
Journal
Neuron
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOUMON Yannick
Tous les auteurs :
Hasan MT, Althammer F, Silva da Gouveia M, Goyon S, Eliava M, Lefevre A, Kerspern D, Schimmer J, Raftogianni A, Wahis J, Knobloch-Bollmann HS, Tang Y, Liu X, Jain A, Chavant V, Goumon Y, Weislogel JM, Hurlemann R, Herpertz SC, Pitzer C, Darbon P, Dogbevia GK, Bertocchi I, Larkum ME, Sprengel R, Bading H, Charlet A, Grinevich V
Lien Pubmed
Résumé
Oxytocin (OT) release by axonal terminals onto the central nucleus of the amygdala exerts anxiolysis. To investigate which subpopulation of OT neurons contributes to this effect, we developed a novel method: virus-delivered genetic activity-induced tagging of cell ensembles (vGATE). With the vGATE method, we identified and permanently tagged a small subpopulation of OT cells, which, by optogenetic stimulation, strongly attenuated contextual fear-induced freezing, and pharmacogenetic silencing of tagged OT neurons impaired context-specific fear extinction, demonstrating that the tagged OT neurons are sufficient and necessary, respectively, to control contextual fear. Intriguingly, OT cell terminals of fear-experienced rats displayed enhanced glutamate release in the amygdala. Furthermore, rats exposed to another round of fear conditioning displayed 5-fold more activated magnocellular OT neurons in a novel environment than a familiar one, possibly for a generalized fear response. Thus, our results provide first evidence that hypothalamic OT neurons represent a fear memory engram.
Référence
Neuron. 2019 May 13;:
