HPV insertional pattern as a personalized tumor marker for the optimized tumor diagnosis and follow-up of patients with HPV-associated carcinomas: a case report.

Date publication

mars 2019

Journal

BMC cancer

Auteurs

Membres identifiés du Cancéropôle Est :
Pr DOLIVET Gilles , Pr HARLE Alexandre , Dr LEROUX Agnès , Pr PEIFFERT Didier , Dr GUILLET-THIBAULT Julie

Tous les auteurs :
Harlé A, Guillet J, Thomas J, Demange J, Dolivet G, Peiffert D, Leroux A, Sastre-Garau X

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/30922253

Résumé

In clinical oncology, only a few applications have been developed using HPV as a personalized tumor marker, a lack most probably related to the limited information obtained by the classical Polymerase Chain Reaction (PCR) approach. To overcome this limitation, we have recently developed the capture-based Next-Generation Sequencing (NGS) "CaptHPV" assay, designed to provide an extensive and comprehensive molecular characterization of HPV DNA sequences associated with neoplasias, ie the sequence of the viral genome (245 genotypes), its physical state, viral load, integration site and genomic alterations at integration locus. These data correspond to highly specific tumor markers that can be used to improve diagnosis and patient's follow-up.

Référence

BMC Cancer. 2019 Mar 28;19(1):277