Fiche publication
Date publication
mars 2019
Journal
Biomaterials
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DUMAS Dominique
Tous les auteurs :
Gluais M, Clouet J, Fusellier M, Decante C, Moraru C, Dutilleul M, Veziers J, Lesoeur J, Dumas D, Abadie J, Hamel A, Bord E, Chew SY, Guicheux J, Le Visage C
Lien Pubmed
Résumé
Annulus fibrosus (AF) impairment is associated with reherniation, discogenic pain, and disc degeneration after surgical partial discectomy. Due to a limited intrinsic healing capacity, defects in the AF persist over time and it is hence necessary to adopt an appropriate strategy to close and repair the damaged AF. In this study, a cell-free biodegradable scaffold made of polycaprolactone (PCL), electrospun, aligned microfibers exhibited high levels of cell colonization, alignment, and AF-like extracellular matrix deposition when evaluated in an explant culture model. The biomimetic multilayer fibrous scaffold was then assessed in an ovine model of AF impairment. After 4 weeks, no dislocation of the implants was detected, and only one sample out of six showed a partial delamination. Histological and immunohistochemical analyses revealed integration of the implant with the surrounding tissue as well as homogeneously aligned collagen fiber organization within each lamella compared to the disorganized and scarcer fibrous tissue in a randomly organized control fibrous scaffold. In conclusion, this biomimetic electrospun implant exhibited promising properties in terms of AF defect closure, with AF-like neotissue formation that fully integrated with the surrounding ovine tissue.
Référence
Biomaterials. 2019 Mar 13;205:81-93
