Fiche publication
Date publication
mars 2019
Journal
Nanomedicine : nanotechnology, biology, and medicine
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOIREAU Wilfrid
,
Dr ELIE-CAILLE Céline
Tous les auteurs :
Obeid S, Sung PS, Le Roy B, Chou ML, Shieh SL, Elie-Caille C, Burnouf T, Boireau W
Lien Pubmed
Résumé
Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVs effective diameter was in the 25-1000nm range, with a majority (≈ 90%)≤100nm. Both T-PEVs in buffer (but not N-PEVs) and non-nanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (> 70nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.
Référence
Nanomedicine. 2019 Mar 13;:
