Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread.

Date publication

mai 2014

Journal

Virology

Auteurs

Membres identifiés du Cancéropôle Est :
Pr MELY Yves , Dr HEINLEIN Manfred

Tous les auteurs :
Niehl A, Pasquier A, Ferriol I, Mély Y, Heinlein M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/24889224

Résumé

Tobacco mosaic virus (TMV) is a longstanding model for studying virus movement and macromolecular transport through plasmodesmata (PD). Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral replication complexes (VRCs) along the ER-actin network towards PD. To investigate whether this movement mechanism might be conserved between tobamoviruses, we compared the functions of Oilseed rape mosaic virus (ORMV) MP with those of MP(TMV). We show that MP(ORMV) supports TMV movement more efficiently than MP(TMV). Moreover, MP(ORMV) localizes to C-MERs like MP(TMV) but accumulates to lower levels and does not localize to larger inclusions/VRCs or along MTs, patterns regularly seen for MP(TMV). Our findings extend the role of C-MERs in viral cell-to-cell transport to a virus commonly used for functional genomics in Arabidopsis. Moreover, accumulation of tobamoviral MP in inclusions or along MTs is not required for virus movement.

Mots clés

Arabidopsis, virology, Endoplasmic Reticulum, metabolism, Inclusion Bodies, Viral, Microtubules, metabolism, Plant Viral Movement Proteins, genetics, Tobamovirus, genetics

Référence

Virology. 2014 May;456-457:43-54