pH-Responsive Nanometric Polydiacetylenic Micelles Allow for Efficient Intracellular siRNA Delivery.

Date publication

novembre 2016

Journal

ACS applied materials & interfaces

Auteurs

Membres identifiés du Cancéropôle Est :
Dr REMY Jean-Serge , Dr WAGNER Alain , Dr KICHLER Antoine , Dr TOUNSI Nassera

Tous les auteurs :
Ripoll M, Neuberg P, Kichler A, Tounsi N, Wagner A, Remy JS

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/27804286

Résumé

A novel generation of pH-responsive photopolymerized diacetylenic amphiphile (PDA) micelles with a diameter of 10 nm was designed and optimized for the intracellular delivery of siRNAs. Dialysis and photopolymerization of the micelles allowed a strong reduction of the cytotoxicity of the nanovector, while the hydrophilic histidine headgroup permitted enhancing the siRNA delivery potential by improving the endosomal escape via imidazole protonation. These PDA-micellar systems were fully characterized by DLS, TEM, and DOSY-NMR experiments. The resulting bioactive complexes of PDA-micelles with siRNA were shown to have an optimal size below 100 nm.

Référence

ACS Appl Mater Interfaces. 2016 Nov;8(45):30665-30670