Fiche publication
Date publication
juin 2006
Journal
The Journal of biological chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CHASSEROT-GOLAZ Sylvette
,
Dr VITALE Nicolas
Tous les auteurs :
de Barry J, Janoshazi A, Dupont JL, Procksch O, Chasserot-Golaz S, Jeromin A, Vitale N
Lien Pubmed
Résumé
Several studies have shown that the neuronal calcium sensor (NCS-1) and phosphoinositol 4-kinase-beta (PI4K-beta) regulate the exocytotic process of nerve and neuroendocrine cells. The aim of our study was to investigate their possible interaction at rest and during stimulation in living cells and to decipher the role of this interaction in the secretory process. In PC12 cells, we observed a stimulation-induced recruitment of NCS-1 and PI4K-beta from the intracellular compartment toward the plasma membrane. This recruitment was highly correlated to the intracellular Ca(2+) rise induced by secretagogues. Using fluorescence resonance energy transfer between PI4K-beta-ECFP and NCS-1-EYFP, we show that both proteins are interacting in resting cells and that this interaction increases with stimulation. It appears that the membrane insertion of NCS-1 is necessary for the interaction with PI4K-beta, since a mutation that prevented the membrane insertion of NCS-1 abolished NCS-1-PI4K-beta interaction, as revealed by fluorescence resonance energy transfer analysis. Additionally, the overexpression of mutated NCS-1 prevents the stimulatory effect on secretion induced by PI4K-beta, suggesting that the interaction of the two proteins on a membrane compartment is necessary for the secretory function. Moreover, extinction of endogenous PI4K-beta by small interfering RNA inhibits secretion and completely prevents the stimulatory effect of NCS-1 on calcium-evoked exocytosis from permeabilized PC12 cells, showing directly for the first time the functional implication of a NCS-1.PI4K-beta complex in regulated exocytosis.
Mots clés
1-Phosphatidylinositol 4-Kinase, genetics, Animals, Calcium, metabolism, Calcium-Binding Proteins, genetics, Cytoplasm, metabolism, Electric Stimulation, Exocytosis, physiology, Gene Expression, Neuronal Calcium-Sensor Proteins, Neurons, cytology, Neuropeptides, genetics, PC12 Cells, Rats, Secretory Vesicles, physiology
Référence
J. Biol. Chem.. 2006 Jun;281(26):18098-111
