Fiche publication
Date publication
mars 2006
Journal
Traffic (Copenhagen, Denmark)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BADER Marie-France
,
Dr CHASSEROT-GOLAZ Sylvette
,
Dr VITALE Nicolas
Tous les auteurs :
Corrotte M, Chasserot-Golaz S, Huang P, Du G, Ktistakis NT, Frohman MA, Vitale N, Bader MF, Grant NJ
Lien Pubmed
Résumé
Phospholipase D (PLD) produces phosphatidic acid (PA), an established intracellular signalling lipid that has been also implicated in vesicular trafficking, and as such, PLD could play multiple roles during phagocytosis. Using an RNA interference strategy, we show that endogenous PLD1 and PLD2 are necessary for efficient phagocytosis in murine macrophages, in line with results obtained with wild-type constructs and catalytically inactive PLD mutants which, respectively, enhance and inhibit phagocytosis. Furthermore, we found that PA is transiently produced at sites of phagosome formation. Macrophage PLD1 and PLD2 differ in their subcellular distributions. PLD1 is associated with cytoplasmic vesicles, identified as a late endosomal/lysosomal compartment, whereas PLD2 localizes at the plasma membrane. In living cells undergoing phagocytosis, PLD1 vesicles are recruited to nascent and internalized phagosomes, whereas PLD2 is only observed on nascent phagosomes. These results provide evidence that both PLD isoforms are required for phagosome formation, but only PLD1 seems to be implicated in later stages of phagocytosis occurring after phagosomal internalization.
Mots clés
Animals, Cell Line, Exocytosis, Humans, Immunoglobulin G, pharmacology, Isoenzymes, immunology, Macrophages, enzymology, Mice, Microscopy, Confocal, Microscopy, Video, Microspheres, Models, Biological, Phagocytosis, immunology, Phagosomes, immunology, Phosphatidic Acids, biosynthesis, Phospholipase D, genetics, RNA Interference, RNA, Messenger, metabolism, Transferrin, metabolism
Référence
Traffic. 2006 Mar;7(3):365-77
