Fiche publication
Date publication
février 1997
Journal
Cellular and molecular neurobiology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BADER Marie-France
,
Dr VITALE Nicolas
Tous les auteurs :
Vitale N, Gonon F, Thiersé D, Aunis D, Bader MF
Lien Pubmed
Résumé
1. Besides having a role in signal transduction, trimeric G proteins may also be involved in membrane trafficking events. In chromaffin cells, G alpha o has been found associated with the membrane of secretory granules. Here we examined the role of Go in regulated exocytosis using pressure microinjection combined with amperometric measurement of catecholamine secretion from individual chromaffin cells. 2. Microinjection of GTP gamma S and mastoparan strongly inhibits the amperometric response to either nicotine or high K+. 3. The presence of mastoparan in the cell incubation medium had no effect on K(+)-evoked secretion, suggesting that mastoparan blocks the exocytotic machinery through an intracellular target protein not located just beneath the plasma membrane. 4. Microinjection of anti-G alpha o antibodies potentiates by more than 50% the K(+)-evoked secretion, whereas anti-G alpha i1/2 antibodies have no effect. 5. Thus an inhibitory Go protein, probably associated with secretory granules, controls exocytosis in chromaffin cells. The intracellular proteins controlling organelle-associated G proteins are currently unknown. The neuronal cytosolic protein GAP-43 stimulates G alpha o in purified chromaffin granule membranes and inhibits exocytosis in permeabilized cells. We show here that microinjection of a synthetic peptide corresponding to the domain of GAP-43 that interacts with Go inhibits secretion. We suggest that GAP-43 or a related cytosolic protein controls the exocytotic priming step in chromaffin, cells by stimulating a granule-associated Go protein.
Mots clés
Antibodies, pharmacology, Catecholamines, secretion, Cells, Cultured, Chromaffin Cells, drug effects, Cytoplasmic Granules, physiology, Electrophysiology, Exocytosis, drug effects, GAP-43 Protein, GTP-Binding Protein alpha Subunits, Gi-Go, GTP-Binding Proteins, immunology, Guanosine 5'-O-(3-Thiotriphosphate), pharmacology, Humans, Membrane Glycoproteins, physiology, Microinjections, Nerve Tissue Proteins, physiology, Nicotine, pharmacology, Peptide Fragments, Peptides, Potassium, pharmacology, Wasp Venoms, pharmacology
Référence
Cell. Mol. Neurobiol.. 1997 Feb;17(1):71-87
